Question

Sterilization of fermentation media with steam is performed at

• A. $100^\circ$C for 30 minutes
• B. $150^\circ$C for 5 minutes
• C. $121^\circ$C for 10 minutes
• D. $121^\circ$C for 20 minutes

Hint:

Autoclave = $121^\circ$C, 15 psi, 15–20 min (standard sterilization condition)

Answer 1

The Correct Option is D

Concept: Sterilization using steam is done by autoclaving, which uses high-pressure saturated steam.

Step 1: Standard conditions.
Autoclaving conditions:
• Temperature: $121^\circ$C
• Pressure: 15 psi
• Time: about 15--20 minutes

Step 2: Analyze options.

• $100^\circ$C → insufficient for sterilization
• $150^\circ$C → not standard autoclaving condition
• $121^\circ$C for 10 min → insufficient time
• $121^\circ$C for 20 min → correct

Step 3: Final conclusion.
Thus, correct sterilization condition is: \[ $121^\circ$C for 20 minutes \]