Question:
Stationary phase for steroidal chromatography is:
Stationary phase for steroidal chromatography is:
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Selecting the correct stationary phase depends on the polarity and nature of the analyte to ensure optimal separation.
Updated On: Jul 14, 2026
- Acylated paper
- Carboxy Paper
- Kieselguhr paper
- Silica Paper
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The Correct Option is A
Approach Solution - 1
- In chromatography, the stationary phase is the phase that does not move and with which the compounds interact.
- Steroidal compounds are generally non-polar and require a stationary phase that can interact effectively to allow separation.
- Acylated paper is paper chemically modified with acyl groups, making it more hydrophobic and suitable for separating steroidal compounds due to better interaction with the non-polar steroids.
- Other options like carboxy paper, kieselguhr paper, and silica paper are typically used for different types of chromatography, such as polar compounds or different analytical purposes.
- Hence, acylated paper is the preferred stationary phase in steroidal chromatography to achieve efficient separation.
- Steroidal compounds are generally non-polar and require a stationary phase that can interact effectively to allow separation.
- Acylated paper is paper chemically modified with acyl groups, making it more hydrophobic and suitable for separating steroidal compounds due to better interaction with the non-polar steroids.
- Other options like carboxy paper, kieselguhr paper, and silica paper are typically used for different types of chromatography, such as polar compounds or different analytical purposes.
- Hence, acylated paper is the preferred stationary phase in steroidal chromatography to achieve efficient separation.
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Approach Solution -2
The question asks which stationary phase is used for steroidal chromatography. Steroids are largely non-polar, so the choice of stationary phase has to be able to interact effectively with a non-polar compound.
- Acylated paper: Ordinary paper is cellulose, which is hydrophilic. Acylating the paper (attaching acyl/fatty acid groups to the cellulose hydroxyls) converts its surface into a hydrophobic layer, which is what is needed to retain non-polar steroids in a reversed-phase partition system.
- Carboxy paper: This modification introduces carboxyl groups, adding polarity and ion-exchange character to the paper, which suits polar or ionic compounds rather than non-polar steroids.
- Kieselguhr paper: Kieselguhr (diatomaceous earth) is a polar, siliceous support typically used to hold a stationary liquid phase in normal-phase or partition chromatography of more polar analytes, not specifically suited to steroid separation.
- Silica paper: Silica is a polar adsorbent, well suited to normal-phase separations of polar compounds, but its polarity works against strong retention of non-polar steroid molecules.
Since steroids need a non-polar surface to partition into, only the acylated (fat-modified) paper provides the right hydrophobic character among the four options.
Therefore, the correct answer is Acylated paper.
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