Question:

Restriction enzyme digestion means

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Restriction digestion = precise cutting of DNA by restriction enzymes.
Updated On: Jul 18, 2026
  • Cutting of DNA
  • Repair of DNA
  • Incubating of DNA
  • Enzymatic digestion of DNA
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The Correct Option is A

Solution and Explanation

Step 1: Understanding restriction enzymes.
Restriction enzymes are proteins that recognize specific DNA sequences and cut DNA at or near these sites.

Step 2: Meaning of digestion.
In molecular biology, digestion refers to breaking down DNA into smaller fragments using restriction enzymes. It does not refer to chemical degradation like in metabolism.

Step 3: Mechanism of action.
These enzymes bind to specific recognition sequences and cleave phosphodiester bonds in DNA strands.

Step 4: Application.
This cutting process is used in cloning, gene mapping, and recombinant DNA technology.

Step 5: Clarifying misconceptions.
It is not repair or incubation; it is precise cutting of DNA at specific sites.

Step 6: Final conclusion.
Thus, restriction enzyme digestion means cutting of DNA.
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