Question

Oxygen limitation in animal cell culture leads to

• A. Reduced cell growth and viability
• B. Increased ATP production
• C. DNA synthesis
• D. Protein folding

Hint:

Remember: \[ \boxed{\text{Low Oxygen} \Longrightarrow \text{Low ATP} \Longrightarrow \text{Reduced Cell Growth}} \] Maintaining dissolved oxygen is one of the most important parameters in animal cell bioreactors.

Answer 1

The Correct Option is A

Concept: Most animal cells require oxygen for aerobic respiration. Oxygen is the final electron acceptor in the electron transport chain and is essential for ATP production. When oxygen becomes limiting, ATP synthesis decreases, leading to reduced cell growth, lower viability, and decreased productivity.

Step 1: Role of oxygen in animal cell culture.
Oxygen is required for:
• ATP production
• Cell growth
• Cell division
• Protein synthesis
• Cell survival Without sufficient oxygen, oxidative phosphorylation slows down.

Step 2: Effects of oxygen limitation.
Low dissolved oxygen results in:
• Reduced ATP generation
• Slower cell growth
• Lower cell viability
• Reduced recombinant protein production
• Increased accumulation of waste metabolites

Step 3: Eliminate incorrect options.

• ATP production decreases rather than increases.
• DNA synthesis slows down under oxygen limitation.
• Protein folding is not the primary consequence.
• The major effect is reduced cell growth and viability. Final Answer: \[ \boxed{Option (A) is correct \]