Question:

Muller Kauffmann Tetrathionate Novobiocin (MKTTn) broth is selective enrichment broth for____ .

Show Hint

Whenever you see "Tetrathionate" or "Selenite" in a broth name in a food microbiology context, the answer is almost certainly Salmonella.
  • Campylobacter
  • Salmonella
  • Clostridium
  • Bacillus
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Question:
The question asks for the target microorganism that is selectively grown and enriched using a specific laboratory medium called MKTTn broth.

Step 2: Detailed Explanation:


Selective Enrichment: In food microbiology, if a pathogen is present in very low numbers or stressed, we use enrichment broths. These inhibit the background microflora while allowing the pathogen to multiply.

Salmonella Detection: The standard ISO method for detecting Salmonella in food involves multiple stages. The enrichment stage often uses two different broths.

MKTTn Broth: Muller-Kauffmann Tetrathionate Novobiocin broth is a highly selective medium designed specifically for the enrichment of {Salmonella species.

How it works: Tetrathionate inhibits common intestinal bacteria (coliforms). The addition of Novobiocin (an antibiotic) specifically inhibits Gram-positive bacteria like Staphylococcus. Brilliant green and Bile salts are also often included to further increase selectivity against competitors.

Step 3: Final Answer:

MKTTn broth is a standard selective enrichment medium for Salmonella.
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