Question

Match the coefficients in List I with their corresponding downstream processing steps in List II.
List I: A. Sedimentation, B. Partition, C. Rejection
List II: I. Aqueous two-phase extraction, II. Ultrafiltration, III. Dialysis, IV. Centrifugation

• A. A-III, B-I, C-IV, D-II
• B. A-II, B-I, C-IV, D-III
• C. A-IV, B-III, C-I, D-II
• D. A-IV, B-I, C-II, D-III

Hint:

Centrifugation = Sedimentation. Extraction = Partition. Membrane = Rejection.

Answer 1

The Correct Option is D

Step 1: Concept
Downstream processing uses physical properties to separate and purify biological products.

Step 2: Meaning
Each separation technique is governed by a specific coefficient.

Step 3: Analysis
- Sedimentation (A) is the basis for Centrifugation (IV). - Partition (B) describes how a solute distributes between two phases, used in Extraction (I). - Rejection (C) describes the exclusion of molecules by a membrane, as in Ultrafiltration (II). - Activity (D) relates to the behavior of solutes in Dialysis (III).

Step 4: Conclusion
The correct matching of process to coefficient is A-IV, B-I, C-II, D-III. Final Answer: (D)