Question

Match List-I with List-II.

List-I	List-II
A. Transformation	I. Restriction enzyme
B. Cloning site	II. Transfer DNA to host bacteria
C. Selection	IV. Antibiotic
D. Ori	III. Replication

Choose the correct answer from the options given below :

• A. A-IV, B-I, C-III, D-II
• B. A-II, B-I, C-IV, D-III
• C. A-I, B-II, C-IV, D-III
• D. A-III, B-IV, C-II, D-I

Hint:

• "Ori" is the direct initiator of Replication, allowing you to link D with III.

• Commonly used antibiotic resistance genes for selection in E. coli vectors include those for ampicillin, chloramphenicol, tetracycline, and kanamycin.

Answer 1

The Correct Option is B

Concept:

• Recombinant DNA technology uses biological elements to clone genes of interest inside host cells.

• Key processes and genetic tools include: itemize

• Transformation: Introducing foreign genetic material directly into a recipient bacterium.

• Cloning Sites: Specific locations on a vector recognized by restriction endonucleases where foreign DNA is inserted.

• Selectable Markers: Genes (such as those providing resistance to antibiotics) used to select recombinant host cells and eliminate non-recombinants.

• Origin of Replication (Ori): A DNA sequence that initiates DNA replication inside host cells.
itemize

Step 1: Establish the matches between List-I and List-II

• A. Transformation: Represents the process used to

II. Transfer DNA to host bacteria.

• B. Cloning site: The physical region in a cloning vector cleaved by a specific

I. Restriction enzyme to insert foreign DNA.

• C. Selection: The process of identifying transformed cells, often using an

IV. Antibiotic resistance gene as a selectable marker.

• D. Ori: The sequence responsible for initiating DNA

III. Replication and controlling the copy number of the plasmid.

Step 2: Match with the given option choices
Combining these correlations, we obtain the pattern: A-II, B-I, C-IV, D-III. This corresponds to option (B).