Question:
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is used for plant cells.
Reason R: Isolation of genetic material needs disruption of cells.
In the light of the above statements, choose the most appropriate answer from the options given below:
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is used for plant cells.
Reason R: Isolation of genetic material needs disruption of cells.
In the light of the above statements, choose the most appropriate answer from the options given below:
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is used for plant cells.
Reason R: Isolation of genetic material needs disruption of cells.
In the light of the above statements, choose the most appropriate answer from the options given below:
Show Hint
Link assertions and reasons using the word "because". "Lysozyme is used for bacteria and cellulase for plants because isolation of genetic material needs cell disruption." Since this makes logical sense, R is the correct explanation.
Memorize the cell wall enzymes: Bacteria \(\rightarrow\) Lysozyme, Plant \(\rightarrow\) Cellulase, Fungi \(\rightarrow\) Chitinase.
Updated On: Jun 21, 2026
- A is not correct but R is correct
- Both A and R are correct and R is the correct explanation of A
- Both A and R are correct but R is not the correct explanation of A
- A is correct but R is not correct
Show Solution
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The Correct Option is B
Solution and Explanation
Concept:
• Recombinant DNA Technology: Requires pure DNA, free from other macromolecules.
• To release DNA, cells must be lysed using specific enzymes to digest cell walls: itemize
• Lysozyme is used for bacterial cell walls (peptidoglycan).
• Cellulase is used for plant cell walls (cellulose).
• Chitinase is used for fungal cell walls (chitin).
itemize
Step 1: Verify Assertion A
Assertion A states that lysozyme breaks down bacterial cells and cellulase breaks down plant cells.
This is true, as these enzymes degrade the biological polymers composing the respective cell walls.
Step 2: Verify Reason R
Reason R states that isolation of genetic material requires cell disruption.
This is true, as the cell membrane and cell wall must be broken to release DNA along with RNA, proteins, polysaccharides, and lipids.
Step 3: Check the explanation link
Why do we use specific enzymes like lysozyme and cellulase? We use them because the first step of isolating DNA requires breaking open the cells (disruption).
Therefore, R is the correct explanation of A.
• Recombinant DNA Technology: Requires pure DNA, free from other macromolecules.
• To release DNA, cells must be lysed using specific enzymes to digest cell walls: itemize
• Lysozyme is used for bacterial cell walls (peptidoglycan).
• Cellulase is used for plant cell walls (cellulose).
• Chitinase is used for fungal cell walls (chitin).
itemize
Step 1: Verify Assertion A
Assertion A states that lysozyme breaks down bacterial cells and cellulase breaks down plant cells.
This is true, as these enzymes degrade the biological polymers composing the respective cell walls.
Step 2: Verify Reason R
Reason R states that isolation of genetic material requires cell disruption.
This is true, as the cell membrane and cell wall must be broken to release DNA along with RNA, proteins, polysaccharides, and lipids.
Step 3: Check the explanation link
Why do we use specific enzymes like lysozyme and cellulase? We use them because the first step of isolating DNA requires breaking open the cells (disruption).
Therefore, R is the correct explanation of A.
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