Question

Match List I with List II
List I (Micropropagation propagules):
A. Protocorms
B. Bulblets
C. Somatic embryos
D. Adventitious shoot buds
List II (Crops):
I. Oil palm
II. Orchids
III. Begonia
IV. Lily
Choose the correct answer from the options given below:

• A. A - II, B - IV, C - III, D - I
• B. A - II, B - I, C - IV, D - III
• C. A - III, B - IV, C - I, D - II
• D. A - II, B - IV, C - I, D - III

Hint:

Orchids are always uniquely associated with protocorms or protocorm-like bodies (PLBs) in micropropagation. Identifying this single match is often enough to eliminate incorrect options immediately.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
Plant tissue culture and micropropagation utilize different types of regenerative structures (propagules) depending on the species and its natural mode of reproduction.
Matching these propagules with their respective target crops helps in designing efficient in vitro multiplication protocols.

Step 2: Detailed Explanation:
Let us analyze each pair in detail:
A. Protocorms: Orchids have tiny seeds without endosperm.
In nature and in vitro, orchid seeds germinate to form a tuber-like structures called protocorms.
In orchid micropropagation, protocorm-like bodies (PLBs) are induced from explants to multiply plants rapidly.
Therefore, A matches with II (Orchids).
B. Bulblets: Lilies are bulbous crops.
In vitro propagation of lilies is most commonly achieved by inducing adventitious bulblets on scale explants.
Therefore, B matches with IV (Lily).
C. Somatic embryos: Somatic embryogenesis is a major pathway for cloning oil palm (Elaeis guineensis).
Because oil palm cannot be propagated by conventional vegetative methods, embryogenic callus is induced from leaf tissues to form somatic embryos, which then germinate into plantlets.
Therefore, C matches with I (Oil palm).
D. Adventitious shoot buds: Begonia is a classic example of a plant with high organogenic capacity.
Excised leaf tissues of Begonia easily form adventitious shoot buds from epidermal cells without an intermediate callus stage.
Therefore, D matches with III (Begonia).

Step 3: Final Answer:
Matching the pairs yields: A - II, B - IV, C - I, D - III, which corresponds to Option (D).