Question

Match List-I with List-II
List-I (Cloning Vector) vs List-II (Characteristic Feature)
(A) Plasmid - (I) Replication restricted to plant gene.
(B) YAC - (II) Replication restricted to mammalian cells.
(C) Cosmid - (III) Replication restricted to bacteria.
(D) Ti Plasmid - (IV) Replication restricted to yeast.
Choose the correct answer from the options given below:

• (A) - (I), (B) - (III), (C) - (IV), (D) - (II)
• (A) - (IV), (B) - (III), (C) - (II), (D) - (I)
• (A) - (III), (B) - (IV), (C) - (II), (D) - (I)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

To easily solve vector matching questions, find the most certain, unique pairs:
- YAC $\rightarrow$ Yeast (IV)
- Ti Plasmid $\rightarrow$ Plant (I)
These two matches are highly specific and allow you to instantly find the correct option.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
Cloning vectors are specialized DNA molecules used to carry foreign genetic material into another cell, where it can be replicated and/or expressed.
Different vectors are designed to replicate within specific host species based on their origin of replication (ori).
Detailed Explanation:
Let us match the vectors in List-I with their corresponding host-specific replication features in List-II:
- (A) Plasmid: Standard plasmids (like pUC19 or pBR322) carry a bacterial origin of replication (such as ColE1) and replicate exclusively within bacterial hosts.
This matches with (III).
- (B) YAC (Yeast Artificial Chromosome): These vectors are designed with yeast centromeres (CEN), telomeres (TEL), and autonomous replicating sequences (ARS) to replicate as linear chromosomes within yeast cells.
This matches with (IV).
- (D) Ti Plasmid (Tumor-inducing plasmid): Obtained from Agrobacterium tumefaciens, this plasmid is used to transfer genetic material (T-DNA) specifically into plant genomes, where it integrates and replicates.
This matches with (I).
- (C) Cosmid: Cosmids are hybrid vectors combining plasmid and lambda phage *cos* sites.
(Note: Although cosmids replicate in bacteria, the remaining option in this match links it to mammalian systems (II) due to the constraints of the test question's structure).
Combining these matches, we get: (A) - (III), (B) - (IV), (C) - (II), (D) - (I).

Step 2: Final Answer:
The matching sequence corresponds to option (C).