Question:
Which of the following are true for a bacterial artificial chromosome?
(A). It is a kind of artificial plasmid.
(B). It can clone a DNA segment of 100000 to 300000 base pairs.
(C). It can clone a DNA segment of 1000000 to 3000000 base pairs.
(D). It can clone a DNA segment of 3000000 to 5000000 base pairs.
Choose the correct answer from the options given below:
Which of the following are true for a bacterial artificial chromosome?
(A). It is a kind of artificial plasmid.
(B). It can clone a DNA segment of 100000 to 300000 base pairs.
(C). It can clone a DNA segment of 1000000 to 3000000 base pairs.
(D). It can clone a DNA segment of 3000000 to 5000000 base pairs.
Choose the correct answer from the options given below:
(A). It is a kind of artificial plasmid.
(B). It can clone a DNA segment of 100000 to 300000 base pairs.
(C). It can clone a DNA segment of 1000000 to 3000000 base pairs.
(D). It can clone a DNA segment of 3000000 to 5000000 base pairs.
Choose the correct answer from the options given below:
Show Hint
To remember vector carrying capacities:
- Plasmid: Up to 10 kb
- Cosmid: 30 to 45 kb
- BAC: 100 to 300 kb
- YAC: 200 to 1000 kb (up to 2 Mb)
- Plasmid: Up to 10 kb
- Cosmid: 30 to 45 kb
- BAC: 100 to 300 kb
- YAC: 200 to 1000 kb (up to 2 Mb)
Updated On: Jul 6, 2026
- (A) and (B) only.
- (B) and (D) only.
- (A) and (C).
- (C) and (D) only.
Show Solution
Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Bacterial Artificial Chromosomes (BACs) are low-copy-number synthetic cloning vectors used to clone and maintain large genomic DNA inserts in E. coli.
Step 2: Detailed Explanation:
- Statement A is correct: A BAC is an artificial plasmid vector constructed using the functional fertility plasmid (F-plasmid) of E. coli. The F-plasmid origins of replication ensure a low copy number (1-2 copies per cell), which helps stabilize large inserts.
- Statement B is correct: BACs are designed to accommodate large inserts, typically ranging from 100 to 300 kilobase pairs (kb), which is equivalent to 100,000 to 300,000 base pairs.
- Statements C and D are incorrect: Insert sizes of 1 to 5 megabases (Mb) are far beyond the packaging limit of BACs. Inserting fragments of this size would cause plasmid instability and prevent uptake during transformation.
- Thus, only statements (A) and (B) are correct.
Step 3: Final Answer:
The correct statements are (A) and (B) only.
Bacterial Artificial Chromosomes (BACs) are low-copy-number synthetic cloning vectors used to clone and maintain large genomic DNA inserts in E. coli.
Step 2: Detailed Explanation:
- Statement A is correct: A BAC is an artificial plasmid vector constructed using the functional fertility plasmid (F-plasmid) of E. coli. The F-plasmid origins of replication ensure a low copy number (1-2 copies per cell), which helps stabilize large inserts.
- Statement B is correct: BACs are designed to accommodate large inserts, typically ranging from 100 to 300 kilobase pairs (kb), which is equivalent to 100,000 to 300,000 base pairs.
- Statements C and D are incorrect: Insert sizes of 1 to 5 megabases (Mb) are far beyond the packaging limit of BACs. Inserting fragments of this size would cause plasmid instability and prevent uptake during transformation.
- Thus, only statements (A) and (B) are correct.
Step 3: Final Answer:
The correct statements are (A) and (B) only.
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