Question

Match List-I with List-II

List-I (Chemical) & List-II (Seed related tests)
(A) $GA_3$ & (II) Seed germination
(B) Thiourea & (IV) Seed dormancy
(C) 2,3,5-triphenyl tetrazolium chloride & (I) Seed viability
(D) Calcium chloride & (III) Seed longevity

• (A) - (II), (B) - (IV), (C) - (III), (D) - (I)
• (A) - (II), (B) - (I), (C) - (IV), (D) - (III)
• (A) - (II), (B) - (IV), (C) - (I), (D) - (III)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

Remember that the Tetrazolium test is the universal biochemical test used to determine Seed Viability (staining live embryos red). Gibberellins (GA3) are the essential phytohormones used to promote Germination.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
Seed testing evaluates various physiological and biochemical properties of seed lots, such as viability, germination rate, dormancy levels, and longevity.
Specific chemical reagents are used to conduct or assist these physiological tests.

Step 2: Detailed Explanation:
Let us match each chemical reagent in List-I with its corresponding seed test in List-II:
- (A) $GA_3$ (Gibberellic Acid) $\rightarrow$ (II) Seed germination:
Gibberellins are the primary plant hormones that trigger and promote seed germination by activating hydrolytic enzymes like alpha-amylase to mobilize stored food reserves.
- (B) Thiourea $\rightarrow$ (IV) Seed dormancy:
Thiourea is a highly effective chemical agent used to break physiological seed dormancy (especially in potato tubers, gladiolus corms, and some weed seeds) by stimulating cellular metabolism.
- (C) 2,3,5-triphenyl tetrazolium chloride $\rightarrow$ (I) Seed viability:
The tetrazolium (TZ) test is the standard rapid chemical test used to determine seed viability.
Living, respirational tissues contain active dehydrogenase enzymes that reduce colorless tetrazolium salt into an insoluble red compound (triphenylformazan), staining viable embryos red.
- (D) Calcium chloride ($\text{CaCl}_2$) $\rightarrow$ (III) Seed longevity:
Calcium chloride is a strong desiccant used in seed storage containers to absorb moisture, maintaining seeds at a low relative humidity, which substantially increases seed longevity.
This results in the sequence: (A)-(II), (B)-(IV), (C)-(I), (D)-(III).

Step 3: Final Answer:
The correct matching sequence is (A) - (II), (B) - (IV), (C) - (I), (D) - (III).