Question

Match List-I with List-II

List-I: Seed Tests & List-II: Purpose
(A). Grow-out test & (I). Leachate discharge
(B). Tetrazolium test & (II). Seed coat permeability
(C). Electrical conductivity & (III). Seed purity
(D). Imbibition test & (IV). Seed viability
Choose the correct answer from the options given below:

• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)
• (A) - (III), (B) - (I), (C) - (IV), (D) - (II)
• (A) - (IV), (B) - (III), (C) - (I), (D) - (II)
• (A) - (IV), (B) - (I), (C) - (II), (D) - (III)

Hint:

Key seed technology takeaways:
- Grow-Out Test: Evaluates Genetic Purity.
- Tetrazolium Test: Evaluates Viability (active respiration turns tissue red).
- Electrical Conductivity: Evaluates Seed Vigor (membrane leakiness).

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
Seed testing is performed to determine the quality, viability, vigor, and genetic purity of seed lots before they are distributed to farmers.

Step 3: Detailed Explanation:
Let us match each seed test with its diagnostic purpose:
- (A). Grow-out test (GOT): This test involves growing a representative sample of a seed lot to maturity and evaluating morphological characteristics.
It is used to assess genetic purity and detect self-pollinated or off-type contaminants, matching (III).
- (B). Tetrazolium test (TZ test): This is a rapid biochemical test.
Living tissues release hydrogen ions during respiration, reducing the colorless 2,3,5-triphenyl tetrazolium chloride dye to a stable, red compound called formazan.
This red staining indicates active respiration, allowing researchers to evaluate seed viability, matching (IV).
- (C). Electrical conductivity (EC) test: High-vigor seeds have intact cell membranes that limit the loss of internal solutes during hydration.
In contrast, seeds with damaged membranes leak electrolytes into the surrounding water.
Measuring the electrical conductivity of this leachate indicates membrane integrity and leachate discharge, matching (I).
- (D). Imbibition test: This test measures the rate of water absorption by seeds over time.
It is used to evaluate seed coat permeability and detect hard seed conditions, matching (II).
This gives the matching: (A) - (III), (B) - (IV), (C) - (I), (D) - (II).

Step 4: Final Answer:
The correct match is Option (A).