Question:
Make the correct sequence of steps involved in the in-vitro mass multiplication of rose variety?
(A). Preparation and inoculation of axillary bud explants
(B). Shoot elongation of plantlets
(C). Rhizogenesis of plantlets
(D). Shoot proliferation of axillary bud explants
(E). Hardening of plantlets
Make the correct sequence of steps involved in the in-vitro mass multiplication of rose variety?
(A). Preparation and inoculation of axillary bud explants
(B). Shoot elongation of plantlets
(C). Rhizogenesis of plantlets
(D). Shoot proliferation of axillary bud explants
(E). Hardening of plantlets
(A). Preparation and inoculation of axillary bud explants
(B). Shoot elongation of plantlets
(C). Rhizogenesis of plantlets
(D). Shoot proliferation of axillary bud explants
(E). Hardening of plantlets
Show Hint
Standard Micropropagation Stages:
Inoculation $\rightarrow$ Shoot Proliferation $\rightarrow$ Shoot Elongation $\rightarrow$ Rooting (Rhizogenesis) $\rightarrow$ Hardening.
Inoculation $\rightarrow$ Shoot Proliferation $\rightarrow$ Shoot Elongation $\rightarrow$ Rooting (Rhizogenesis) $\rightarrow$ Hardening.
Updated On: Jul 29, 2026
- (A), (B), (D), (E), (C)
- (A), (D), (B), (C), (E)
- (D), (B), (E), (C), (A)
- (D), (E), (A), (C), (B)
Show Solution
Verified By Collegedunia
The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept:
In-vitro mass propagation (micropropagation) is a plant tissue culture technique used to rapidly multiply clonal, disease-free plants on a commercial scale.
For woody ornamentals like roses, micropropagation is carried out through nodal segment or axillary bud culture.
The process is divided into distinct, sequential stages to ensure successful establishment, multiplication, rooting, and acclimatization of the plants.
Step 2: Detailed Explanation:
Let us arrange the given steps in their correct chronological sequence for rose micropropagation:
Step 1: Preparation and inoculation of axillary bud explants (A):
Nodal segments containing axillary buds are collected from healthy mother plants.
These explants are surface-sterilized using disinfectants (like sodium hypochlorite or mercuric chloride) and inoculated onto a sterile, nutrient-rich agar medium (usually MS medium) under aseptic conditions in a laminar airflow chamber.
Shoot proliferation of axillary bud explants (D):
The inoculated buds are incubated under controlled light and temperature.
Under the influence of high cytokinin levels in the medium, the axillary buds are induced to proliferate, producing multiple shoot clumps.
Step 2: Shoot elongation of plantlets (B):
The proliferated shoot clumps are transferred to an elongation medium (with a lower cytokinin-to-auxin ratio) to allow the individual shoots to grow and elongate to a suitable height.
Step 3: Rhizogenesis of plantlets (C):
The elongated shoots are dissected and transferred to a rooting medium containing auxins (such as IBA or NAA) to induce root initiation and development (rhizogenesis).
Step 5: Hardening of plantlets (E):
The rooted plantlets (micro-shoots with roots) are transferred from the sterile, high-humidity in-vitro environment to a greenhouse or shade net house in a soil-less medium (like coco peat and perlite).
This hardening process allows the plants to develop a cuticular wax layer and adapt to natural humidity and temperature before field planting.
This sequence corresponds to (A), (D), (B), (C), (E), matching option (B).
Step 3: Final Answer:
Therefore, the correct sequential order of steps is (B).
In-vitro mass propagation (micropropagation) is a plant tissue culture technique used to rapidly multiply clonal, disease-free plants on a commercial scale.
For woody ornamentals like roses, micropropagation is carried out through nodal segment or axillary bud culture.
The process is divided into distinct, sequential stages to ensure successful establishment, multiplication, rooting, and acclimatization of the plants.
Step 2: Detailed Explanation:
Let us arrange the given steps in their correct chronological sequence for rose micropropagation:
Step 1: Preparation and inoculation of axillary bud explants (A):
Nodal segments containing axillary buds are collected from healthy mother plants.
These explants are surface-sterilized using disinfectants (like sodium hypochlorite or mercuric chloride) and inoculated onto a sterile, nutrient-rich agar medium (usually MS medium) under aseptic conditions in a laminar airflow chamber.
Shoot proliferation of axillary bud explants (D):
The inoculated buds are incubated under controlled light and temperature.
Under the influence of high cytokinin levels in the medium, the axillary buds are induced to proliferate, producing multiple shoot clumps.
Step 2: Shoot elongation of plantlets (B):
The proliferated shoot clumps are transferred to an elongation medium (with a lower cytokinin-to-auxin ratio) to allow the individual shoots to grow and elongate to a suitable height.
Step 3: Rhizogenesis of plantlets (C):
The elongated shoots are dissected and transferred to a rooting medium containing auxins (such as IBA or NAA) to induce root initiation and development (rhizogenesis).
Step 5: Hardening of plantlets (E):
The rooted plantlets (micro-shoots with roots) are transferred from the sterile, high-humidity in-vitro environment to a greenhouse or shade net house in a soil-less medium (like coco peat and perlite).
This hardening process allows the plants to develop a cuticular wax layer and adapt to natural humidity and temperature before field planting.
This sequence corresponds to (A), (D), (B), (C), (E), matching option (B).
Step 3: Final Answer:
Therefore, the correct sequential order of steps is (B).
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