Question

is the medium used for sterility testing as per Indian Pharmacopoeia.

• A. Nutrient broth
• B. Nutrient agar
• C. Meat extract
• D. Fluid thioglycollate medium

Hint:

Thioglycollate = Anaerobes. Soyabean Casein = Aerobes/Fungi. These are the "Big Two" for sterility.

Answer 1

The Correct Option is D

Step 1: Concept
Sterility testing is performed to ensure that pharmaceutical products are free from viable microorganisms.

Step 2: Meaning
The IP specifies two primary media: Fluid Thioglycollate Medium (FTM) and Soyabean Casein Digest Medium (SCDM).

Step 3: Analysis
Fluid Thioglycollate Medium is primarily used for the detection of anaerobic bacteria, though it can also support aerobic growth. Soyabean Casein Digest is used for fungi and aerobic bacteria.

Step 4: Conclusion
Among the given options, Fluid thioglycollate medium is the official medium used for sterility testing.

Final Answer: (D)