Question:
Information about the isoelectric point will be useful in the purification of a protein by which of the following chromatography techniques?
Information about the isoelectric point will be useful in the purification of a protein by which of the following chromatography techniques?
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• pI → Charge of protein
• Charge-based separation → Ion exchange chromatography
Updated On: May 13, 2026
- Gel Filtration chromatography
- Ion exchange chromatography
- Reverse phase chromatography
- Hydrophobic interaction chromatography
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The Correct Option is B
Solution and Explanation
Concept:
The isoelectric point (pI) is the pH at which a protein has no net charge.
• Below pI → Protein is positively charged
• Above pI → Protein is negatively charged
Step 1: Understanding chromatography principles.
• Gel filtration: Separation based on size → pI not relevant.
• Ion exchange chromatography: Separation based on charge → depends on pI → Relevant.
• Reverse phase chromatography: Based on hydrophobicity → pI not directly used.
• Hydrophobic interaction chromatography: Based on hydrophobic interactions → pI not primary factor.
Step 2: Selecting the correct answer.
Since pI determines the charge of protein, it is most useful in ion exchange chromatography.
• Below pI → Protein is positively charged
• Above pI → Protein is negatively charged
Step 1: Understanding chromatography principles.
• Gel filtration: Separation based on size → pI not relevant.
• Ion exchange chromatography: Separation based on charge → depends on pI → Relevant.
• Reverse phase chromatography: Based on hydrophobicity → pI not directly used.
• Hydrophobic interaction chromatography: Based on hydrophobic interactions → pI not primary factor.
Step 2: Selecting the correct answer.
Since pI determines the charge of protein, it is most useful in ion exchange chromatography.
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