Question

In which method of Hb estimation is hemoglobin converted specifically into carboxyhemoglobin?

• A. Sahli's Method
• B. Cyanmethemoglobin Method
• C. Haldane Method
• D. Tallqvist Method

Hint:

{Haldane Method} – Hemoglobin forms {carboxyhemoglobin}.
{Sahli Method} – Hemoglobin forms {acid hematin}.
{Cyanmethemoglobin Method} – Most accurate modern Hb estimation technique.

Answer 1

The Correct Option is C

Concept: Hemoglobin estimation is an important laboratory test used to measure the concentration of hemoglobin in blood. Different methods are based on converting hemoglobin into specific derivatives that can be measured colorimetrically. In the Haldane Method, hemoglobin combines with carbon monoxide to form carboxyhemoglobin, which produces a stable color that can be measured.
Step 1: Identify the method where hemoglobin forms carboxyhemoglobin.

• Sahli's Method – Converts hemoglobin to acid hematin.
• Cyanmethemoglobin Method – Converts hemoglobin to cyanmethemoglobin.
• Haldane Method – Converts hemoglobin to carboxyhemoglobin.
• Tallqvist Method – Visual color comparison method.

Thus, the method in which hemoglobin is converted into carboxyhemoglobin is the Haldane Method.