Question:

In tissue culture, most popular medium used is MS medium. Here, MS refers to the ____________.

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MS medium has relatively high salt concentrations compared to other media like Woody Plant Medium (WPM), which is often preferred for woody or tree species.
  • Manganese Sulfide Medium
  • Murashige & Skoog medium
  • Multiple Shoot producing medium
  • Miller & Smith medium
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Concept:
Plant tissue culture relies on synthetic nutrient media containing essential macro- and micro-elements, vitamins, amino acids, and carbon sources.
This supports the growth of plant cells in vitro.

Step 2: Detailed Explation:

The most famous, versatile, and widely used basal formulation is the Murashige and Skoog (MS) medium.
It was formulated in 1962 by two plant physiologists, Toshio Murashige and Folke K. Skoog, during their research on tobacco tissue culture.
MS medium is characterized by its high concentrations of mineral salts, particularly ammonium and nitrate nitrogen, which support vigorous vegetative growth.
This formulation has proved successful for the micropropagation, organogenesis, and callus induction of a wide range of monocotyledonous and dicotyledonous plant species, including forest trees.

Step 3: Fil Answer:

MS refers to the Murashige & Skoog medium in plant tissue culture.
Hence, the correct option is (B).
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