Question:

Identify the step, in which phosphorylation occurs with the help of inorganic phosphate and not ATP.

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Look for the oxidation step in which inorganic phosphate is added while NAD is reduced.
Updated On: Nov 12, 2026
  • Glucose (6C) 1M \(\rightarrow\) Glucose -6- phosphate (6C) 1M
  • Glyceraldehyde-3-phosphate (3C) \(\rightarrow\) 1,3 bisphosphoglycerate (3C)
  • 2 Phosphoenol pyruvate (3C) \(\rightarrow\) Pyruvic acid (3C)
  • 1,3 bisphosphoglycerate (3C) \(\rightarrow\) 3 Phosphoglycerate (3C)
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Concept
Some glycolytic steps use ATP as the phosphate donor, some give phosphate to ADP, and one uses free inorganic phosphate.

Step 2: Check each step
Glucose to glucose-6-phosphate takes phosphate from ATP.\n1,3-bisphosphoglycerate to 3-phosphoglycerate and phosphoenol pyruvate to pyruvate give phosphate to ADP.

Step 3: Identify the step
When glyceraldehyde-3-phosphate is oxidised by its dehydrogenase, NAD is reduced and inorganic phosphate is attached to form 1,3-bisphosphoglycerate.

Final Answer:
Glyceraldehyde-3-phosphate to 1,3 bisphosphoglycerate uses inorganic phosphate. \[ \boxed{\text{(B) }\text{Glyceraldehyde-3-phosphate (3C)} \rightarrow \text{1,3 bisphosphoglycerate (3C)}} \]
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