Question

Identify the incorrect statement related to get electrophoresis

• A. Separated DNA fragments can be directly seen under UV radiation
• B. Separated DNA can be extracted from get piece
• C. Fragment of DNA moves toward anode
• D. Sieving effect of agarose gel helps in separation of DNA fragments

Answer 1

The Correct Option is A

The question pertains to the topic of gel electrophoresis, a laboratory technique used for the separation of DNA, RNA, or proteins based on their size and charge. Let's evaluate each statement to identify the incorrect one:

1. Separated DNA fragments can be directly seen under UV radiation: This statement is incorrect. DNA fragments themselves are not visible under UV light. They must be stained with a fluorescent dye like ethidium bromide, which binds to the DNA and fluoresces under UV light, making the DNA visible.
2. Separated DNA can be extracted from gel piece: This statement is correct. After separation, specific DNA fragments can be cut out from the gel and extracted (a process known as gel extraction) for further experiments.
3. Fragment of DNA moves toward anode: This statement is correct. DNA fragments are negatively charged due to their phosphate backbone. In an electric field, they migrate towards the positive electrode, or anode.
4. Sieving effect of agarose gel helps in separation of DNA fragments: This statement is correct. The agarose gel acts as a molecular sieve, separating DNA fragments based on size as smaller fragments move faster through the pores of the gel than larger ones.

Therefore, the statement "Separated DNA fragments can be directly seen under UV radiation" is incorrect, as visualization requires staining with a fluorescent dye.