Question

Identify the correct sequence of steps in Hershey and Chase's experiment.

• A. Infection \(\rightarrow\) Centrifugation \(\rightarrow\) Blending \(\rightarrow\) Detection of radioactivity.
• B. Centrifugation \(\rightarrow\) Infection \(\rightarrow\) Blending \(\rightarrow\) Detection of radioactivity
• C. Infection \(\rightarrow\) Blending \(\rightarrow\) Centrifugation \(\rightarrow\) Detection of radioactivity
• D. Blending \(\rightarrow\) Infection \(\rightarrow\) Centrifugation \(\rightarrow\) Detection of radioactivity

Hint:

Think of the order in which bacteria were infected, agitated and spun down.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept
Hershey and Chase labelled phages with \(^{32}\text{P}\) (DNA) and \(^{35}\text{S}\) (protein).

Step 2: Key Point
The steps are: infect bacteria with labelled viruses, blend to remove the viral coats from the bacterial surface, centrifuge to separate the bacteria from the coats, and then detect the radioactivity.

Step 3: Compare the options
So the order is infection, blending, centrifugation, detection of radioactivity.

Final Answer:
Infection, blending, centrifugation and then detection is the right order. \[ \boxed{\text{(C) }\text{Infection }\rightarrow\text{ Blending }\rightarrow\text{ Centrifugation }\rightarrow\text{ Detection of radioactivity}} \]