Question:
High cytokinin concentration in the tissue culture media will lead to
High cytokinin concentration in the tissue culture media will lead to
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High Cytokinin:Auxin ratio = Shoot regeneration; Low Cytokinin:Auxin ratio = Root regeneration; Intermediate ratio = Callus formation.
Updated On: Jul 13, 2026
- Apical dominance
- Inhibition of apical dominance
- Leaf growth
- Root growth
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept:
Organogenesis in plant tissue culture is primarily regulated by the balance between two phytohormone groups: auxins and cytokinins.
Step 2: Detailed Explanation:
Skoog and Miller established that the ratio of auxin to cytokinin determines the developmental pathway of explants:
- A high auxin-to-cytokinin ratio promotes root initiation and development (rhizogenesis).
- A high cytokinin-to-auxin ratio promotes shoot bud differentiation and growth (caulogenesis).
Cytokinins stimulate cell division in the shoot apical meristems and release lateral buds from the inhibitory effects of auxins, directly counteracting apical dominance.
In shoot tip or nodal cultures, incorporating high concentrations of cytokinins in the nutrient medium inhibits apical dominance, stimulating lateral bud outgrowth and shoot proliferation.
Step 3: Final Answer:
A high cytokinin concentration in tissue culture media leads to the inhibition of apical dominance and shoot proliferation.
Organogenesis in plant tissue culture is primarily regulated by the balance between two phytohormone groups: auxins and cytokinins.
Step 2: Detailed Explanation:
Skoog and Miller established that the ratio of auxin to cytokinin determines the developmental pathway of explants:
- A high auxin-to-cytokinin ratio promotes root initiation and development (rhizogenesis).
- A high cytokinin-to-auxin ratio promotes shoot bud differentiation and growth (caulogenesis).
Cytokinins stimulate cell division in the shoot apical meristems and release lateral buds from the inhibitory effects of auxins, directly counteracting apical dominance.
In shoot tip or nodal cultures, incorporating high concentrations of cytokinins in the nutrient medium inhibits apical dominance, stimulating lateral bud outgrowth and shoot proliferation.
Step 3: Final Answer:
A high cytokinin concentration in tissue culture media leads to the inhibition of apical dominance and shoot proliferation.
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