Question:

Given below are two statements :
Statement I : Bacterial plasmids can be used as a vector for gene transfer in plants.
Statement II : Plasmids are double stranded, extrachromosomal DNA found in bacteria which are capable of autonomous replication in suitable host.
In light of the above statements, choose the correct answer from the options given below.

Show Hint

To serve as efficient cloning vectors, engineered plasmids must contain three essential components: a origin of replication (ori), a selectable marker gene (like antibiotic resistance), and a multiple cloning site (MCS).
  • Both Statement (I) and Statement (II) are true.
  • Both Statement (I) and Statement (II) are false.
  • Statement (I) is true but Statement (II) is false.
  • Statement (I) is false but Statement (II) is true.
Show Solution
collegedunia
Verified By Collegedunia

The Correct Option is A

Solution and Explanation

Step 1: Understanding the Concept:
Plasmids are small, circular DNA molecules found in bacteria that are physically separate from chromosomal DNA.
Because they are easy to manipulate and can replicate independently, they are valuable tools in molecular biology and genetic engineering.

Step 2: Detailed Explanation:

Let us analyze both statements:
- Statement (I): Bacterial plasmids are widely used as vectors to introduce foreign genes into plant genomes.
The most famous example is the Tumor-inducing (Ti) plasmid from the soil bacterium Agrobacterium tumefaciens.
By replacing the disease-causing genes on the Ti plasmid with a target gene, scientists can use the plasmid's natural machinery to transfer and integrate recombinant DNA into plant cells.
Thus, Statement (I) is true.
- Statement (II): Plasmids are physically defined as double-stranded, circular, extrachromosomal DNA molecules found in bacteria and some yeast.
They contain their own origin of replication (ori), which allows them to replicate autonomously within a suitable host cell.
Thus, Statement (II) is true.
Consequently, both Statement (I) and Statement (II) are true.

Step 3: Final Answer:

Both Statement (I) and Statement (II) are true, corresponding to option (A).
Was this answer helpful?
0
0

Top ICAR AIEEA Plant Biotechnology Questions

View More Questions