Question:
Germinative capacity (viability) of Barley is normally tested by -
Germinative capacity (viability) of Barley is normally tested by -
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The tetrazolium test is a highly valued rapid test in seed technology because it can determine the viability of a seed lot within a few hours, bypassing the days required for a traditional germination shelf trial.
Updated On: Aug 20, 2026
- Caramel staining
- Malachite green staining
- Tetrazolium staining
- Alum staining
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept:
For industrial malting, it is essential to evaluate the percentage of living, viable barley grains capable of germinating.
A rapid biochemical test is preferred over slow natural germination trials.
Step 2: Detailed Explanation:
The viability (germinative capacity) of barley is standardly evaluated using Tetrazolium staining (the TTC test):
- The grains are cut longitudinally and soaked in a solution of 2,3,5-triphenyltetrazolium chloride (TTC).
- In living, metabolically active embryonic tissues, active dehydrogenase enzymes reduce the colorless soluble TTC salt into a stable, water-insoluble, bright red compound called formazan.
- Consequently, viable embryos stain a deep red color, while dead embryos remain uncolored.
Step 3: Final Answer:
The correct method is Tetrazolium staining, which corresponds to option (C).
For industrial malting, it is essential to evaluate the percentage of living, viable barley grains capable of germinating.
A rapid biochemical test is preferred over slow natural germination trials.
Step 2: Detailed Explanation:
The viability (germinative capacity) of barley is standardly evaluated using Tetrazolium staining (the TTC test):
- The grains are cut longitudinally and soaked in a solution of 2,3,5-triphenyltetrazolium chloride (TTC).
- In living, metabolically active embryonic tissues, active dehydrogenase enzymes reduce the colorless soluble TTC salt into a stable, water-insoluble, bright red compound called formazan.
- Consequently, viable embryos stain a deep red color, while dead embryos remain uncolored.
Step 3: Final Answer:
The correct method is Tetrazolium staining, which corresponds to option (C).
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