Question:
Enzymes that join foreign DNA to plasmids to form recombinant DNA are:
Enzymes that join foreign DNA to plasmids to form recombinant DNA are:
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DNA ligase acts as molecular glue in genetic engineering.
Updated On: Jun 20, 2026
- DNA ases
- Primases
- Exonucleases
- DNA ligases
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The Correct Option is D
Solution and Explanation
Step 1: Understanding recombinant DNA formation.
Recombinant DNA technology involves cutting DNA and joining DNA fragments from different sources.
Step 2: Role of ligase enzyme.
DNA ligase forms phosphodiester bonds between DNA fragments, sealing breaks in the DNA backbone.
Step 3: Function in plasmid recombination.
After restriction enzymes cut DNA, ligase joins foreign DNA with plasmid DNA to form stable recombinant DNA.
Step 4: Evaluating other enzymes.
- DNAases: degrade DNA.
- Primase: synthesizes RNA primers.
- Exonuclease: removes nucleotides from ends.
Step 5: Logical reasoning.
Only ligase can join DNA fragments permanently.
Step 6: Final conclusion.
Thus, DNA ligase is the correct answer.
Recombinant DNA technology involves cutting DNA and joining DNA fragments from different sources.
Step 2: Role of ligase enzyme.
DNA ligase forms phosphodiester bonds between DNA fragments, sealing breaks in the DNA backbone.
Step 3: Function in plasmid recombination.
After restriction enzymes cut DNA, ligase joins foreign DNA with plasmid DNA to form stable recombinant DNA.
Step 4: Evaluating other enzymes.
- DNAases: degrade DNA.
- Primase: synthesizes RNA primers.
- Exonuclease: removes nucleotides from ends.
Step 5: Logical reasoning.
Only ligase can join DNA fragments permanently.
Step 6: Final conclusion.
Thus, DNA ligase is the correct answer.
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