Question

Entrapment of enzyme involves

• A. Binding enzyme to surface
• B. Chemical modification
• C. Denaturation
• D. Enclosing enzyme in a matrix

Hint:

Remember the immobilization methods: \[ \boxed{\text{Entrapment}=\text{Enzyme enclosed inside a gel or matrix}} \] The enzyme stays inside the matrix while substrates and products move through the pores.

Answer 1

The Correct Option is D

Concept: Enzyme immobilization is a technique in which enzymes are confined so that they can be reused while retaining their catalytic activity. One important method is entrapment, where enzymes are physically enclosed within a gel or polymer matrix without forming covalent bonds.

Step 1: Understand enzyme entrapment.
During entrapment, enzymes remain inside a porous matrix while substrates and products freely diffuse through the pores. Common matrices include:
• Calcium alginate
• Polyacrylamide
• Agar
• Carrageenan

Step 2: Advantages of entrapment.
Entrapment offers several advantages:
• Enzymes remain active.
• Easy recovery and reuse.
• Increased enzyme stability.
• Reduced contamination of products.

Step 3: Eliminate incorrect options.

• Binding enzymes to a surface refers to adsorption.
• Chemical modification changes enzyme structure.
• Denaturation destroys enzyme activity.
• Entrapment specifically means enclosing enzymes within a matrix. Final Answer: \[ \boxed{Option (D) is correct \]