Step 1: Understanding the Concept
During replication helicase opens the helix, topoisomerase relieves strain and DNA polymerase builds new strands.
Step 2: Role of ligase
The lagging strand is made as Okazaki fragments with nicks between them.
DNA ligase seals the nicks with phosphodiester bonds, so the new strand is continuous and the daughter duplex is complete.
Step 3: Why the other options fail
Permease transports lactose, helicase only unwinds DNA and topoisomerase removes twisting strain.
Final Answer:
The enzyme is DNA ligase.
\[ \boxed{\text{(B) }\text{ligase}} \]