Question:
During PCR cycle, the temperature range in the step of annealing is __________.
During PCR cycle, the temperature range in the step of annealing is __________.
Show Hint
Annealing is the cooler middle step of a PCR cycle, between denaturation and extension.
Updated On: Nov 12, 2026
- \(40-60\ ^{\circ }\text{C}\)
- \(70-75\ ^{\circ }\text{C}\)
- \(90-98\ ^{\circ }\text{C}\)
- \(65-85\ ^{\circ }\text{C}\)
Show Solution
Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept
Each PCR cycle has three steps: denaturation, annealing and extension.
Step 2: Temperatures
Denaturation is at about \(90-98\ ^{\circ}\text{C}\), which separates the DNA strands. Annealing is at about \(40-60\ ^{\circ}\text{C}\), where primers pair with the template. Extension is at about \(70-75\ ^{\circ}\text{C}\), where Taq polymerase extends the primers.
Step 3: Conclusion
So annealing occurs at \(40-60\ ^{\circ}\text{C}\).
Final Answer:
Annealing takes place at 40-60 degrees Celsius. \[ \boxed{\text{(A) }40-60\ ^{\circ}\text{C}} \]
Each PCR cycle has three steps: denaturation, annealing and extension.
Step 2: Temperatures
Denaturation is at about \(90-98\ ^{\circ}\text{C}\), which separates the DNA strands. Annealing is at about \(40-60\ ^{\circ}\text{C}\), where primers pair with the template. Extension is at about \(70-75\ ^{\circ}\text{C}\), where Taq polymerase extends the primers.
Step 3: Conclusion
So annealing occurs at \(40-60\ ^{\circ}\text{C}\).
Final Answer:
Annealing takes place at 40-60 degrees Celsius. \[ \boxed{\text{(A) }40-60\ ^{\circ}\text{C}} \]
Was this answer helpful?
0
0
Top MHT CET Biology Questions
- During r- DNA technology, which one of the following enzymes is used for cleaving DNA molecule ?
- Important sperm lysin used for dissolving egg covering is
- In biogas plant, the role of acidogenic bacteria is to
- In $C_{3}$ plants, the first stable product of photosynthesis during the dark reaction is
- In case of anaerobic respiration, the RQ is ___________
View More Questions
Top MHT CET Recombinant DNA technology and applications Questions
- When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of \rule{2cm{0.15mm} enzyme.}
- Which of the following techniques are used for the transfer of foreign DNA into the host cell?
(A). Microinjection
(B). Shot gun
(C). Ultrasonification
(D). Gel permeation
(E). Electroporation
Choose the correct answer from the options given below: - Identify the correct statements regarding biotechnology.
(A). Exonucleases cut the nucleotides at the ends of DNA strands.
(B). The sequences recognized by restriction enzymes are 4 to 8 nucleotides long.
(C). Baculoviruses are not vectors.
(D). Agrobacterium tumefaciens is a bacterium which can live only in plants.
(E). The bacteria protect their own DNA from nuclease attack by methylating the bases at susceptible sites.
Choose the correct answer from the options given below: - Which of the following characteristics, a good cloning vector must possess, to be efficiently used in rDNA technology?
(A). It should have the ability of independent replication.
(B). It should have marker genes for antibiotic resistance.
(C). It should be able to easily be introduced into host cells.
(D). It should have suitable control elements like promoter, operator, ribosome binding sites, etc.
Choose the correct answer from the options given below: - Which enzyme is used for inserting foreign DNA into the vector DNA in recombinant DNA technology?
View More Questions
Top MHT CET Questions
- During r- DNA technology, which one of the following enzymes is used for cleaving DNA molecule ?
- In non uniform circular motion, the ratio of tangential to radial acceleration is (r = radius of circle, $v =$ speed of the particle, $\alpha =$ angular acceleration)
- The temperature of $32^{\circ}C$ is equivalent to
- The line $5x + y - 1 = 0$ coincides with one of the lines given by $5x^2 + xy - kx - 2y + 2 = 0 $ then the value of k is
- The heat of formation of water is $ 260\, kJ $ . How much $ H_2O $ is decomposed by $ 130\, kJ $ of heat ?
View More Questions