Question

DNA inserted into a ________ can be tested or manipulated in two different cell types.

• A. \( \text{Phasmid} \)
• B. \( \text{Transfer vector} \)
• C. \( \text{Shuttle vector} \)
• D. \( \text{Phagemid} \)

Hint:

The most common shuttle vectors are the YEp (Yeast Episomal Plasmids) which "shuttle" between E. coli and Yeast.

Answer 1

The Correct Option is C

Concept: Vectors are DNA molecules used as vehicles to carry foreign genetic material into another cell.
• Standard vectors are usually designed for one specific host (like E. coli).
• Some complex experiments require moving the DNA between two unrelated species.

Step 1: The Dual Nature of Shuttle Vectors.
A shuttle vector is a vector constructed so that it can propagate in two different host species. For this to be possible, the vector must contain two different origins of replication (ori). One "ori" allows it to replicate in a prokaryote (like bacteria), and the other allows it to replicate in a eukaryote (like yeast or mammalian cells).

Step 2: Purpose of the Shuttle.
Researchers often use bacteria for easy manipulation and cloning of the DNA. They then "shuttle" the DNA into a eukaryotic cell to test the protein expression or functional effects. Final Answer: DNA inserted into a Shuttle vector can be tested or manipulated in two different cell types.