Question:
Arrange the steps of tissue culture (used for largescale multiplication of superior genotypes) in the correct order:
A). Acclimatisation
B). Establishment of aseptic culture
C). Selection of media and growth regulator
D). Root Induction
E). Multiple shoot production
Arrange the steps of tissue culture (used for largescale multiplication of superior genotypes) in the correct order:
A). Acclimatisation
B). Establishment of aseptic culture
C). Selection of media and growth regulator
D). Root Induction
E). Multiple shoot production
A). Acclimatisation
B). Establishment of aseptic culture
C). Selection of media and growth regulator
D). Root Induction
E). Multiple shoot production
Show Hint
Acclimatisation (A) is always the fil step of any micropropagation protocol, as it involves preparing laboratory-grown plants for the harsh outdoor environment.
Updated On: Jul 28, 2026
- A, B, E, C, D
- A, C, B, E, D
- E, D, C, B, A
- C, B, E, D, A
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The Correct Option is D
Solution and Explanation
Step 1: Understanding the Concept:
Plant tissue culture (micropropagation) is a laboratory technique used to clone superior genotypes (plus trees) rapidly under controlled, sterile conditions.
The process follows a sequential path from media preparation and explant inoculation to shoot multiplication, rooting, and field acclimation.
Step 2: Detailed Explation:
Let us trace the logical and technical sequence of micropropagation:
- Step 1 (Selection of media and growth regulator - C): Before initiating the culture, suitable basal media (such as MS, Woody Plant Medium, etc.) and optimal concentrations of growth regulators (auxins and cytokinins) must be formulated.
- Step 2 (Establishment of aseptic culture - B): The selected explant (shoot tip, nodal segment) is surface-sterilized and inoculated onto the medium to establish a contamition-free, healthy culture.
- Step 3 (Multiple shoot production - E): Cytokinins are adjusted to promote auxiliary or adventitious bud proliferation, producing multiple shoots.
- Step 4 (Root Induction - D): Individual shoots are transferred to a rooting medium containing auxins to induce root formation.
- Step 5 (Acclimatisation - A): The rooted plantlets are gradually hardened off to survive tural field conditions by transitioning from high humidity in vitro to ambient conditions.
Thus, the correct chronological sequence is C, B, E, D, A.
Step 3: Fil Answer:
The correct order of steps in tissue culture is C, B, E, D, A.
Hence, the correct option is (D).
Plant tissue culture (micropropagation) is a laboratory technique used to clone superior genotypes (plus trees) rapidly under controlled, sterile conditions.
The process follows a sequential path from media preparation and explant inoculation to shoot multiplication, rooting, and field acclimation.
Step 2: Detailed Explation:
Let us trace the logical and technical sequence of micropropagation:
- Step 1 (Selection of media and growth regulator - C): Before initiating the culture, suitable basal media (such as MS, Woody Plant Medium, etc.) and optimal concentrations of growth regulators (auxins and cytokinins) must be formulated.
- Step 2 (Establishment of aseptic culture - B): The selected explant (shoot tip, nodal segment) is surface-sterilized and inoculated onto the medium to establish a contamition-free, healthy culture.
- Step 3 (Multiple shoot production - E): Cytokinins are adjusted to promote auxiliary or adventitious bud proliferation, producing multiple shoots.
- Step 4 (Root Induction - D): Individual shoots are transferred to a rooting medium containing auxins to induce root formation.
- Step 5 (Acclimatisation - A): The rooted plantlets are gradually hardened off to survive tural field conditions by transitioning from high humidity in vitro to ambient conditions.
Thus, the correct chronological sequence is C, B, E, D, A.
Step 3: Fil Answer:
The correct order of steps in tissue culture is C, B, E, D, A.
Hence, the correct option is (D).
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