Question

Along with Histone like DNA binding proteins which enzyme maintains supercoiled state in packaging of DNA in prokaryotes?

• A. DNA polymerase and RNA primase
• B. DNA ligase and helicase
• C. DNA gyrase and topoisomerase I
• D. Phosphorylase and RNA polymerase

Hint:

Topoisomerases such as DNA gyrase and topoisomerase I control the supercoiled state of bacterial DNA.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept
Prokaryotes lack true histones, so their DNA is folded in loops with histone-like proteins and kept supercoiled.

Step 2: Identify the enzymes
Supercoiling is controlled by topoisomerases.
DNA gyrase adds negative supercoils and topoisomerase I removes excess ones.

Step 3: Why the other options fail
DNA polymerase, primase, ligase, helicase, phosphorylase and RNA polymerase work in replication, transcription or metabolism, not in supercoiling.

Final Answer:
DNA gyrase and topoisomerase I maintain the supercoiled state. \[ \boxed{\text{(C) }\text{DNA gyrase and topoisomerase I}} \]