Question:
All the following can determine the protein structure except
All the following can determine the protein structure except
Updated On: Feb 10, 2026
- Mass spectrometry
- X-ray crystallography
- High performance liquid chromatography
- NMR spectroscopy
Show Solution
Verified By Collegedunia
The Correct Option is C
Solution and Explanation
To determine the three-dimensional (3D) structure of proteins, specialized analytical techniques are employed. Let's review each option and see why or why not it is suitable for determining protein structures:
- Mass spectrometry: Mass spectrometry is primarily used for determining the mass, identify the protein, and analyzing the quantity of proteins. It is not typically used to directly determine the 3D structure of proteins.
- X-ray crystallography: This is a widely used method to determine the 3D atomic structure of proteins. By measuring the patterns of X-rays diffracted by a crystallized sample of the protein, scientists can construct a detailed map of electron densities within the protein, thus deducing its structure.
- High performance liquid chromatography (HPLC): HPLC is mainly used for separating, identifying, and quantifying components in a mixture. It is not used for determining the 3D structure of proteins. Therefore, this is the correct answer, as it cannot determine protein structure.
- NMR spectroscopy: Nuclear Magnetic Resonance (NMR) spectroscopy can be used to determine the structure of proteins in solution by measuring the magnetic properties of atomic nuclei. It provides information on the 3D arrangement of atoms in the protein.
The correct answer is therefore High performance liquid chromatography, as this technique is not involved in determining the 3D structure of proteins but rather in separating and identifying components of mixtures.
Was this answer helpful?
0
0
Top GPAT Organic Chemistry Questions
- Given below are the two statements
Statement I: Glucose‐6‐phosphate can be formed from glucose, but not from glycogen.
Statement II: Glucose‐1‐phosphate may be hydrolyzed to yield free glucose in liver.
Choose the most appropriate answer from the options given below: - Optically active mandelic acid can be synthesized from benzaldehyde in the presence of the enzyme:
- In the determination of configuration of glucose, Fischer subjected (‐) arabinose to Ruff’s degradation. The four‐carbon sugar obtained by the degradation process was:
- Insertion of a oxygen in a carbonyl compound to form an ester is known as:
- Controlled alkylation of a ketone via an enamine intermediate is named as:
View More Questions
Top GPAT Amino Acids Questions
- Optically active mandelic acid can be synthesized from benzaldehyde in the presence of the enzyme:
- Amine group protection during peptide synthesis can be accomplished by converting the amine to
- This Compound 2,5,8,11,14,17,20,23,26-nona-oxaoctacosan-28-yl p-(butylamino) benzoate is usually known for its activity?
- Gabriel ring closure method is employed for the synthesis of :
- Which among the following is a non-essential amino acid?
View More Questions
Top GPAT Questions
- Match the symbols in the List I with the terms used in the List II of conductance measurements, and
List I
SymbolsList II
TermsA \(\Omega^{-1}\) I Specific conductance B \(∧\) II Electrical conductance C k III Specific resistance D \(\rho\) IV Equivalent conductance
Choose the correct answer from the options given below: - Cottrell’s method is used for the measurement of:
- Match the statements in List I with their correct answers in List II, respectively, in respect to modified 1st law of thermodynamics, and
List I
List II
A Constant heat (q = 0) I Isothermal B Reversible process at constant temperature (dT = 0) II Isometric C Constant volume (dV = 0) III Adiabatic D Constant pressure (dP = 0) IV Isobar
Choose the correct answer from the options given below: - Boric acid with molecular weight 61.83 was partitioned between water and amyl alcohol at 25 °C. The amount of boric acid was determined to be 0.24 g in 250 ml of amyl alcohol and 0.32 g in 100 ml water. The partition coefficient of boric acid between water and amyl alcohol, when calculated at molar concentration for each of the solution, is:
- Drugs ‘A’, ‘B’ and ‘C’ follow zero order, first order and second order degradation kinetics, respectively, but have the same rate constant. Which of the following statement is true in this respect:
View More Questions