List of top Life Science Questions

Select the correct order of steps involved in Cloning:
A. Selection
B. Restriction digestion
C. Ligation
D. Transformation
E. Gene of Interest (Insert) and vector

Select the correct order of steps of plant tissue culture:
A. Explant sterilization
B. Selection of media
C. Excision of regenerated shoots
D. Inoculation of explants
E. Transfer of plantlets to sterilised soil

Select the correct order of the steps involved in Gram staining:
A. Counter staining
B. Crystal violet- Iodine (CV-I)
C. Heat Fixation
D. Decolorizing agent

For identification of desired D by Southern blotting, select the correct order
A. Blotting
B. Probe binding
C. Autoradiogram
D. Electrophoresis
E. Gel treatment

For transport of acyl CoA into mitochondria, select the correct order of steps involved:
A. Acyl- carnitine to acyl CoA
B. Acyl- carnitine transported to mitochondrial matrix
C. Acyl group of acyl CoA transferred to carnitine
D. Carnitine released and return to cytosol

Arrange the following in order 5' - 3' direction
A. Promoter
B. UTR5'
C. ORF
D. Termitor

Select the correct sequence of steps involved in TCA cycle:
A. $\alpha$-Ketoglutarate
B. Succinyl CoA
C. Fumarate
D. Citrate
E. Oxaloacetate

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Leaves exposed to the Sun, are thinner, have larger area of lami have more chlorophyll and less stomata.
Reason R: Plants become structurally and physiologically adapted to the amount of solar radiations received.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Nervous and Endocrine Systems act together to coordite functions of all body systems.
Reason R: Responses of the endocrine system are often slower but their influence is broader and they regulate all types of body cells.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Cardiac excitation normally begins in the sinoatrial (SA) node.
Reason R: SA node cells do not have a stable resting potential. They depolarize to threshold spontaneously.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Father with mitochondrial gene defect cannot transmit the disease to his offspring.
Reason R: Mitochondria are the cell organelles which contain D.

Match the LIST-I with LIST-II

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Electron transport chain (ETC) and ATP synthesizing system are located on the inner mitochondrial membrane.
Reason R: The inner surface of the inner mitochondrial membrane possess phosphorylating subunits.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Bovine serum albumin (BSA) is not immunogenic when injected into cow but is strongly immunogenic when injected in rabbit.
Reason R: The greater the phylogenetic distance between two species, the less the structural disparity between them.

Select the correct sequence of morphological changes occurring in Apoptosis
A. Membrane blebbing
B. Phagocytosis
C. Nuclear fragmentation
D. Apoptotic bodies

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: The amino acid sequences of Histones H3 and H4 are not highly conserved.
Reason R: Nearly all of the amino acids in a histone molecule are engaged in an interaction with either D or another histone.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: 12-20% acrylamide in PAGE restricts the migration of large molecules.
Reason R: Pore size of the gel affects the separation of molecules.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: X chromosome is acrocentric and Y chromosome is submetacentric.
Reason R: This is based on the position of centromere on the chromosome.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Spectroscopy technique is used for the study of interaction of electromagnetic radiation with matter.
Reason R: It is used for the identification of substances through the spectrum emitted or absorbed by them.

Match the LIST-I with LIST-II

The first sound (S$_1$) 'lubb' is caused by blood turbulence associated with:

Sexual dimorphism is seen in:

Genetic drift is of significance in:

The photochemical reaction of photosynthesis occurs in which part of chloroplast?

D polymerase $\delta$ in eukaryotes is involved in: