Step 1: Understanding the Question:
The question asks to match different physical separation methods of chromatography in LIST-I with their corresponding separation principles in LIST-II. Step 2: Key Formula or Approach:
Match each chromatographic technique to its defining separation principle:
• Gas Chromatography (GC): Separates thermally stable volatile or volatilized compounds.
• Affinity Chromatography: Separates molecules based on specific biological ligand-receptor binding interactions.
• Gel-Filtration (Size-Exclusion) Chromatography: Separates biomolecules according to molecular size/hydrodymic volume.
• Ion-Exchange Chromatography (IEC): Separates charged molecules based on net electrostatic interactions with charged matrix groups. Step 3: Detailed Explation:
• A. Gas Chromatography $\to$ IV. Separation of volatile components: GC uses a gaseous mobile phase to transport volatilized alytes through a coated column.
• B. Affinity Chromatography $\to$ III. Separation of biomolecules with different binding specificities: Relies on specific bioaffinity interactions (e.g., enzyme-substrate, antibody-antigen).
• C. Gel-Filtration Chromatography $\to$ II. Separation based on size of molecules: Uses porous beads; larger molecules elute first as they cannot enter pores, while smaller molecules enter pores and elute later.
• D. Ion-Exchange Chromatography $\to$ I. Separation of ionic solutes: Uses cationion exchange resins to separate charged species. Step 4: Fil Answer:
The correct matching sequence is A-IV, B-III, C-II, D-I.
