List of top Plant Biotechnology Questions asked in ICAR AIEEA (PG)

Which of the following subunit of RNA polymerase complex is absent during elongation phase of bacterial transcription?
Which of the following is not related to termination of a transcript in E. coli?
What are isoaccepting tRNAs?
The consensus sequence 5'-ACCAUGG-3'; is also known as
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The CAM plants undergo night time acidification and day time deacidification.
Reason (R) : The CAM plants take up $\text{CO}_2$ during day time, store it in the form of malate in vacuoles and break it down to release $\text{CO}_2$ in the night time.
Match List-I with List-II

List-I (Mineral) & List-II (Deficiency Symptom)
(A) Manganese & (IV) Poor wound healing due to the inactivation of Prolidase enzyme
(B) Selenium & (III) Enhanced oxidative stress
(C) Magnesium & (II) Poor Vitamin D status due to lesser formation of biologically
& active form of Vitamin D
(D) Molybdenum & (I) Neurological abnormalities due to sulfite oxidase deficiency

Characteristics of bundle sheath cells in $\text{C}_4$ plants}
(A) Have thin cell walls
(B) Contain centrifugally arranged chloroplasts
(C) Contain stacked thylakoid membranes and do not have starch granules
(D) Carry out light-independent reactions of photosynthesis
In a nucleotide sequence ATGC, which of the following nucleotide has the unlinked 5' - OH?
Which of the following metabolites is not an intermediate in the triglyceride biosynthesis by Kennedy pathway?
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The free histidine is a dominant buffering system at physiological pH than the protein-bound or dipeptide histidine.
Reason (R) : In proteins or dipeptides, the imidazole pKa decreases substantially-in combination with other amino acids- to a level which is more than 1 pH unit removed from intracellular pH.
Match List-I with List-II

List-I (Name of the lipid) & List-II (Nature of the lipid)
(A) Ceramide & (I) Ether glycerophospholipid
(B) Cerebroside & (IV) Neutral Glycosphingolipid
(C) Ganglioside & (II) Acidic (charged) glycosphingolipid
(D) Platelet Activating Factor & (III) Structural parent of all sphingolipids

The synthesis and/or activity of the nitrogenase enzyme gets stimulated by which of the following conditions?
Which of the following interventions would result in enhanced chromatographic separation?
(A) Increasing the number of theoretical plates
(B) Decreasing the Height Equivalent to a Theoretical Plate (HETP)
(C) Decreasing the column height
(D) Decreasing the size of the particles used to pack a column
Match List-I with List-II

List-I (Name of the component) & List-II (Vitamin/Coenzyme)
(A) Pantothenic acid & (II) Coenzyme A
(B) Para-Amino Benzoic Acid (PABA) & (III) Folic acid
(C) Ribose & (I) NAD
(D) Pentanoic acid & (IV) Lipoic acid

Which of the following amino acids in a protein or peptide does not contribute significantly towards its UV absorption at 280 nm?
Some of the exceptional properties of regulatory/allosteric enzymes are:
(A) Their Kinetics do not obey the Michaelis-Menten equation.
(B) They are mostly monomeric in nature.
(C) They have more than one substrate binding sites and the substrate binding to different sites is mutually independent, exclusive and noncooperative.
(D) Binding of effector molecules to them may lead to their activation or inhibition.
Which of the following regions of Nitrate reductase (NR) enzyme is extremely important for the 14-3-3 protein-mediated posttranslational regulation of its activity?
The pyruvate-phosphate dikinase (PPDK) is a key enzyme of photosynthesis and catalyses a reaction similar to the one catalyzed by the pyruvate kinase (PK)-a glycolytic enzyme. Which of the following statements about these two enzymes is true?
The energetic efficiency (in terms of number of ATP equivalents per absorbed photon) of non-cyclic electron transport process in photosynthesis, after taking into account the ATPs yielded by NADPH produced in it, is:
The chemical identity of Oxygen Evolving Centers (OEC) present in the Photosytem-II of plants and cyanobacteria is:
Match List-I with List-II

List-I (Inhibitor/Redox carrier in ETC) & List-II (Action)
(A) Amytal & (I) Inhibits FAD-linked oxidation
(B) Antimycin & (II) Inhibits $\text{NAD}^+$-linked oxidation
(C) Tetramethyl-p-phenylenediamine & (III) Completely inhibits oxidation of all
(TMPD) & electron donors
(D) Sodium azide & (IV) Transfers electrons directly to
& Cytochrome C
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The "Randle Cycle" describes the inhibition of the glycolysis by fatty acid oxidation.
Reason (R) : Oxidation of fatty acids increases the concentration of citrate that in turn inhibits the phosphofructokinase enzyme of glycolytic pathway.
In light of the above statements, choose the correct answer from the options given below.
The first three steps of the $\beta$-oxidation pathway of fatty acyl-CoA, chemically resemble the citric acid cycle reactions carrying out which of the following biochemical conversion?
The catalytic center of which of the following protease families consists of a catalytic triad of aspartate, histidine and serine ?
Match List-I with List-II

List-I (Name of the enzyme) & List-II (Role in the fatty acid oxidation)
(A) 2,4-dienoyl-CoA reductase & (I) Involved in the $\alpha$-oxidation of branched fatty acids
(B) Phytanoyl- CoA hydroxylase & (II) Involved in the oxidation of unsaturated fatty acids
(C) Acyl-CoA oxidase & (III) Involved in the $\omega$-oxidation of medium chain fatty acids
(D) Cytochrome P450 & (IV) Involved in the peroxisomal $\beta$-oxidation