List of top Questions asked in ICAR AIEEA (PG)- 2022

Plasmid DNA isolation methods are primarily based on the
A transgenic event is differentiated from other events by
Function of ligase is
A) to repair single-stranded breaks that arise in double-stranded DNA
B) to join together two individual fragments of double stranded DNA
Choose the correct answer from the options given below:
Agar, the gelling agent used for tissue culture media, is obtained from
Plasmid vector will essentially have
Which of the restriction enzyme does produce fragments with blunt end upon restriction digestion?
Alkaline phosphatase is used to
Genetic engineering involves the following steps
A. Gene manipulation and vector construction
B. Selection and confirmation of the transformants
C. Gene isolation
D. Genetic transformation
What is the correct sequence in which these steps are performed?
What types of DNA molecules are copied using rolling circle replication process?
Which of the following is NOT a millet crop?
Joining two DNA molecules using an adapter involves the following steps
A. Artificial synthesis of an adapter
B. Treating with polynucleotide kinase
C. Blunt end ligation to add an adapter to a blunt-ended DNA molecule
D. Joining two DNA molecules with sticky ends
What is the correct sequence in which these steps are performed? [Question ID = 658][Question Description = 158_34_PLB_AUG22_Q58]
Which of the following is NOT correct for vacuole? [Question ID = 654][Question Description = 154_34_PLB_AUG22_Q54]
Which of the following statement(s) are correct in describing the gene in DNA?
Statement I: The start/initiator codon for the genes can be found on the \(5^{\prime} \rightarrow 3^{\prime}\) strand of DNA
Statement II: The start/initiator codon for the genes can be found on the \(3^{\prime} \rightarrow 5^{\prime}\) strand of DNA
Choose the correct answer from the options given below: [Question ID = 656][Question Description = 156_34_PLB_AUG22_Q56]
Bt brinjal was developed for resistance to [Question ID = 659][Question Description = 159_34_PLB_AUG22_Q59]
Which of the following usually have less dependency on nitrogenous fertilizer for its commercial cultivation? [Question ID = 655][Question Description = 155_34_PLB_AUG22_Q55]
Which of the following is also known as golden fibre? [Question ID = 660][Question Description = 160_34_PLB_AUG22_Q60]
Somatic embryogenesis is a desired method of plant regeneration for plant transformation because [Question ID = 657][Question Description = 157_34_PLB_AUG22_Q57]
Match List I with List II
List I
A. Gene
B. Jumping gene
C. Cistron
D. Rolling circle \(\theta\)X DNA replication
List II
I. Barbara McClintock
II. Johannsen
III. Benzer
IV. Dressler
V. Cairns
Choose the correct answer from the options given below: [Question ID = 650][Question Description = 150_34_PLB_AUG22_Q50]
The major groups of multicellular eukaryotes - plants, fungi and animals - each evolved from a different group of the eukaryotes generally referred to as protists.
A. Most protists are unicellular.
B. Chloroplast containing protists gave rise to plants.
C. Multicellularity evolved in several protists lineages.
D. Archaea are also protists
Choose the correct answer describing Protists from the options given below: [Question ID = 652][Question Description = 152_34_PLB_AUG22_Q52]
Bacterial conjugation can lead to gain of new alleles in recipient cell
Statement I: Through involving crossing over between DNA fragment of donor and recipient cell
Statement II: Through gain of plasmid from donor bacterium to recipient bacterium
In light of the above statements, choose the correct answer from the options given below: [Question ID = 649][Question Description = 149_34_PLB_AUG22_Q49]
Blue-white colonies screening of transformed E. coli cells involved disruption of which of the following gene of cloning vector? [Question ID = 653][Question Description = 153_34_PLB_AUG22_Q53]
Which of the following is correct statement for RNA synthesis?
A. A single RNA polymerase can synthesize all types of RNA in prokaryotes
B. RNA polymerase II can synthesize mRNA in eukaryotes
C. A single RNA polymerase can synthesize all types of RNA in eukaryotes
D. RNA polymerase II can synthesize all types of RNA in prokaryotes
E. E. coli cell has only one type of RNA polymerase
Choose the correct answer from the options given below: [Question ID = 648][Question Description = 148_34_PLB_AUG22_Q48]
Match List I with List II
List I
A. Functional genomics
B. Gene inactivation
C. In-vivo protein-protein interaction
D. Identification of unknown protein
List II
I. Homologous recombination
II. Protein-Protein interaction
III. Mass spectrometry
IV. Bimolecular Fluorescence Complementation
V. Yeast 2-hybrid assay
Choose the correct answer from the options given below: [Question ID = 651][Question Description = 151_34_PLB_AUG22_Q51]
Which of the following technique(s) is used for protein-protein interaction studies? [Question ID = 644][Question Description = 144_34_PLB_AUG22_Q44]
Which of the following is required for wet-heat sterilization in plant tissue culture experiments? [Question ID = 642][Question Description = 142_34_PLB_AUG22_Q42]