List of top Questions asked in GATE BT- 2026

Which one of the following options represents the products of light reactions of photosynthesis in plants?
You have purified an enzyme using a series of chromatographic methods. It was observed that a 10 \(\mu\)g mL-1 of this purified enzyme converted 10 mM substrate per hour at 25\(^{\circ}\)C and pH 7. Its specific activity is IU \(\mu\)g-1. (rounded off to three decimal places)
The equation \(\dfrac{d^2y}{dx^2} - y = 0\) has a solution of the form \(y = e^{Ax}\). The value(s) of \(A\) satisfying this is/are:
In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?
The mean of the following series is . (rounded off to three decimal places)
\(0.01,\ 0.02,\ 0.03,\ \ldots,\ 1\)
Which of the following drugs inhibit(s) ATP-ADP translocase?
A straight line \(y = x - 1\) intersects a circle with center at \(x = 1, y = 1\) and radius of magnitude 1 at two points. The length of the chord formed by this intersection is ________. (rounded off to three decimal places)
You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is 1800 \(\mu\)moles L-1 min-1 and the total concentration of the enzyme in the reaction mixture is 1.5 \(\mu\)M, then the turnover number of the enzyme is s-1. (answer in integer)
The electron equivalent per liter (\(e^{-}\)eq L\(^{-1}\)) of acetate ion solution of concentration 10 g L\(^{-1}\) is \(e^{-}\)eq L\(^{-1}\). (rounded off to one decimal place)
Match the immunological terms in Column I with their function/description in Column II.

Column IColumn II
P. J gene1. Suppresses immune system by blocking calcineurin
Q. Cyclosporin2. Antibody binding site on a large molecule
R. Epitope3. Small foreign molecule which elicits immune response only when combined with a carrier molecule
S. Hapten4. Contributes to antibody diversity
"He often the numbers. False claims are not going to help. Honesty trust", said the manager.
Choose the option with the correct order of words to fill the blanks.
Which of the following is/are posttranslational modification(s) involved in epigenetic control of gene expression?
From the following plot of \(\dfrac{dy}{dx}\) versus \(x\), and if \(y(2) = 5\), the value of \(y(3)\) is . (rounded off to one decimal place)
The RNA primer synthesized during bacterial DNA replication is removed by .
As shown in the figure, circle \(C_1\) with center \(O_1\) and radius \(r_1\) touches the square \(VWXY\) at points \(P\) and \(Q\) while circle \(C_2\) with center \(O_2\) and radius \(r_2\) touches the square \(VWXY\) at points \(R\) and \(S\). The two circles touch each other at \(T\).
Given \(r_1 = 1\) cm and \(\overline{VY} = \overline{VW} = 4\) cm, \(r_2 =\) cm.
Match the biomolecules in Column I with their function given in Column II.

Column IColumn II
P. Lectins1. Acts as ATP dependent pump to efflux out small molecules
Q. P-glycoprotein2. Targets lysosomal enzymes to their destination
R. Digitoxigenin3. Specific carbohydrate binding proteins
S. Mannose 6-phosphate4. Inhibits Na+-K+ pump
A microbial culture is being grown in a bioreactor with continuous aeration. The dissolved oxygen probe provided with the system shows a reading of zero. In this context, which of the following inferences is/are correct?
The RNA sequence below depicts the part of a 330 nucleotides long mRNA and it encodes the C-terminal portion of a protein.
5'-... ... ... AAC ACC ACG ACC CAU GUG GCG AGA CGG UAG-3'
A mutation was identified in this RNA denoted as \(322A \rightarrow U\). This nucleotide change is represented by which of the following class(es) of mutation?
In the sequence of tiles shown below, the missing tile indicated by the question mark should be
A packed bed bioreactor with length 1 m and inside diameter 10 cm has liquid flowing at an interstitial velocity of 1 cm s\(^{-1}\). Given a volumetric flow rate of 0.025 L s\(^{-1}\), the void fraction of the packed bed is . (rounded off to two decimal places)
A substrate is consumed in a zero order reaction such that its concentration falls from 42 g L\(^{-1}\) to 14 g L\(^{-1}\) in 4 hours. The total time taken for complete utilization of substrate will be hours. (answer in integer)
The transfer function of a Proportional-Integral (PI) controller \(G_C(s)\) is given by
\[ G_C(s) = K_C\left(1 + \frac{1}{\tau_I s}\right) \]
where \(K_C\) is the controller gain, \(\tau_I\) is the controller integral time constant and \(s\) is the Laplace variable. The role of the integral component of the controller is to .
In an aerobic fermentation with air sparging, which of the following options can be used to increase the volumetric mass transfer coefficient for oxygen transfer?
Consider the two functions \(f_1(x) = \dfrac{x^2-4}{x-2}\) and \(f_2(x) = x^2 - 2x + 2\). Which of the following is the value of \((f_1(x)+f_2(x))\) as \(x \to 2\)?
A heat exchanger during operation in a bioprocess has a steady temperature of 90 \(^{\circ}\)C. After completion of its operation, it was shut down and it was observed that the rate of decrease of temperature at any time was directly proportional to the difference \(T(t) - 30\) \(^{\circ}\)C, where \(T(t)\) denotes temperature at time \(t\). It was observed that it took 30 min for the temperature to drop to 70 \(^{\circ}\)C. The temperature after 51.5 min will be ________ \(^{\circ}\)C. (rounded off to the nearest integer)