Question

Which vector can carry the largest DNA inserts?

• A. Plasmid
• B. Cosmid
• C. BAC
• D. YAC

Hint:

Vector capacity (increasing order): \[ \boxed{ \text{Plasmid} < \text{Cosmid} < \text{BAC} < \text{YAC} } \]

Answer 1

The Correct Option is D

Step 1: Recall the cloning capacities of common vectors. Different cloning vectors can accommodate different sizes of foreign DNA. \[ \boxed{ \begin{aligned} \text{Plasmid} &\rightarrow 2\text{--}10\ \text{kb} \text{Cosmid} &\rightarrow 35\text{--}45\ \text{kb} \text{BAC} &\rightarrow 100\text{--}300\ \text{kb} \text{YAC} &\rightarrow 200\text{--}1000\ \text{kb} \end{aligned} } \]

Step 2: Identify the vector with the highest capacity. Among the given vectors, \[ \boxed{\text{Yeast Artificial Chromosome (YAC)}} \] can accommodate the largest DNA inserts. Therefore, \[ \boxed{(D)} \] is the correct answer.