Match the following
\[
\begin{array}{|c|l|c|l|}
\hline
& Disease / Application & & Molecular basis / Use
\hline
A & \text{Sickle cell anemia} & I & \text{Frameshift mutation}
B & \text{Thalassemia} & II & \text{Missense point mutation}
C & \text{Duchenne muscular dystrophy} & III & \beta\text{-globin gene defect}
D & \text{PCR} & IV & \text{DNA amplification technique}
\hline
\end{array}
\]
The correct matching is