Question:
Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?
Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?
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- CD and FTIR → secondary structure.
- X-ray → full 3D structure.
- Mass spec → composition, not structure.
- X-ray → full 3D structure.
- Mass spec → composition, not structure.
Updated On: Aug 26, 2025
- Circular dichroism spectroscopy
- Fourier transform infrared spectroscopy
- Mass spectrometry
- X-ray crystallography
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The Correct Option is C
Solution and Explanation
Step 1: Protein secondary structure (α-helix, β-sheet) can be determined by CD spectroscopy and FTIR, as they measure characteristic absorption patterns.
Step 2: X-ray crystallography provides 3D structural information, including secondary structure.
Step 3: Mass spectrometry is powerful for protein identification, sequencing, and modifications, but not for direct determination of secondary structure content.
Final Answer: Mass spectrometry cannot be used for secondary structure determination.
\[\boxed{\text{Correct Answer: (C)}}\]
Step 2: X-ray crystallography provides 3D structural information, including secondary structure.
Step 3: Mass spectrometry is powerful for protein identification, sequencing, and modifications, but not for direct determination of secondary structure content.
Final Answer: Mass spectrometry cannot be used for secondary structure determination.
\[\boxed{\text{Correct Answer: (C)}}\]
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