Question

Which one of the following is NOT a technique used for direct transfer of foreign DNA into the protoplast of host cell without using vector?

• A. Electroporation
• B. Gel electrophoresis
• C. ultrasonication
• D. Lipofection

Hint:

Direct gene transfer techniques put DNA into cells without a vector, whereas gel electrophoresis only separates DNA fragments.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
Vectorless or direct gene transfer includes electroporation, ultrasonication (sonoporation), lipofection and microinjection.

Step 2: Check the techniques
Electroporation uses electric pulses to make pores, lipofection uses liposomes, and ultrasonication uses sound waves.

Step 3: Compare the options
Gel electrophoresis is used to separate DNA fragments by size, so it is not a gene transfer method.

Final Answer:
Gel electrophoresis is not a technique for direct transfer of DNA. \[ \boxed{\text{(B) }\text{Gel electrophoresis}} \]