Question

Which one of the following is NOT a technique used for isolation and characterization of macromolecules?

• A. Gel electrophoresis.
• B. Electrophoresis.
• C. Polymerase Chain Reaction (PCR).
• D. Spectroscopy.

Hint:

Separation methods and spectroscopy study macromolecules. Decide which method only makes copies of DNA.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept
Macromolecules such as DNA and proteins are separated by electrophoresis, gel electrophoresis and chromatography. They are characterised by methods such as spectroscopy.

Step 2: Role of PCR
PCR does not separate or analyse a molecule. It makes many copies of a chosen DNA segment by repeated denaturation, annealing and extension.

Step 3: Conclusion
So PCR is not a technique for isolation and characterisation of macromolecules.

Final Answer:
PCR is not used for isolation and characterisation of macromolecules. \[ \boxed{\text{(C) }\text{Polymerase Chain Reaction (PCR).}} \]