>
Exams
>
Biotechnology
>
Molecular Biology
>
which of the following techniques uses temperature
Question:
Which of the following techniques uses temperature cycling for DNA amplification?
Show Hint
In PCR, temperature cycling is a key step that enables DNA amplification through denaturation, annealing, and extension.
AP PGECET - 2025
AP PGECET
Updated On:
Jun 18, 2025
DNA fingerprinting
PCR
RAPD
Northern blotting
Hide Solution
Verified By Collegedunia
The Correct Option is
B
Solution and Explanation
Polymerase Chain Reaction (PCR) uses repeated cycles of heating and cooling to amplify specific DNA sequences. This temperature cycling is essential for denaturing the DNA, annealing primers, and extending the DNA strands.
Download Solution in PDF
Was this answer helpful?
0
0
Top Questions on Molecular Biology
What is the standard sequence of steps in a PCR (Polymerase Chain Reaction) cycle?
CUET (PG) - 2026
Life Science
Molecular Biology
View Solution
Which technique is specifically used to detect the presence of a specific DNA sequence in a sample?
CUET (PG) - 2026
Life Science
Molecular Biology
View Solution
In gel electrophoresis of DNA, toward which electrode do the DNA fragments migrate?
CUET (PG) - 2026
Life Science
Molecular Biology
View Solution
Which type of bond characterizes the primary structure of DNA?
CUET (PG) - 2026
Bioinformatics
Molecular Biology
View Solution
Which molecular technique is used to detect specific mRNA molecules in a sample?
CUET (PG) - 2026
Bioinformatics
Molecular Biology
View Solution
View More Questions
Questions Asked in AP PGECET exam
The absolute humidity of air at 101.325 kPa is measured to be 0.02 kg of water per kg of dry air. Then the partial pressure of water vapour in the air is:
AP PGECET - 2025
Thermodynamics
View Solution
If the vapour pressure at two temperatures of a solid phase in equilibrium with its liquid phase is known, then the latent heat of fusion can be calculated by the:
AP PGECET - 2025
Thermodynamics
View Solution
1 mole of Argon gas is heated at constant pressure from 200 K to 600 K.
If \( C_p = 4\ \text{cal} \cdot \text{deg}^{-1} \cdot \text{mol}^{-1} \), then the change in entropy will be:
(Given \( \ln 3 = 1.09 \))
AP PGECET - 2025
Thermodynamics
View Solution
The minimum amount of work required to operate a refrigerator which removes 1000 Cal heat at $0^\circ$C and rejects at $50^\circ$C will be:
AP PGECET - 2025
Thermodynamics
View Solution
The entropy of single crystalline Silicon at absolute zero will be:
AP PGECET - 2025
Thermodynamics
View Solution
View More Questions