Question

Which of the following technique is used for invitro amplification of gene?

• A. Electrophoresis
• B. PCR
• C. PAGE
• D. SDS-PAGE

Hint:

Amplification of DNA in a test tube uses repeated cycles with a thermostable DNA polymerase.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
Polymerase Chain Reaction (PCR) makes many copies of a target DNA segment in vitro.

Step 2: Other techniques
Electrophoresis, PAGE and SDS-PAGE are used to separate nucleic acids or proteins by size and charge, not to amplify DNA.

Step 3: Compare the options
PCR is the amplification technique.

Final Answer:
PCR is used for in vitro amplification of a gene. \[ \boxed{\text{(B) }\text{PCR}} \]