Question:
Which of the following methods is used tentatively to separate/identify non-volatile compound(s) present in spice/herb?
Which of the following methods is used tentatively to separate/identify non-volatile compound(s) present in spice/herb?
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Remember:
- Volatile components (essential oils) \(\rightarrow\) analyzed by GC.
- Non-volatile components (pungent principles, pigments) \(\rightarrow\) analyzed by HPLC.
- Volatile components (essential oils) \(\rightarrow\) analyzed by GC.
- Non-volatile components (pungent principles, pigments) \(\rightarrow\) analyzed by HPLC.
Updated On: Aug 20, 2026
- High-performance liquid chromatography (HPLC)
- Distillation
- Gas chromatography (GC)
- Steam-distillation
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Analytical separation techniques are selected based on the volatility and thermal stability of the target chemical compounds.
Step 2: Detailed Explanation:
- Non-Volatile/Heat-Sensitive Compounds: These cannot be easily vaporized without chemical decomposition.
Therefore, Gas Chromatography (GC) and distillation methods cannot be used.
High-Performance Liquid Chromatography (HPLC) is the premier analytical technique used to separate and identify non-volatile compounds (such as capsaicin in chillies, curcumin in turmeric, and piperine in black pepper) dissolved in a liquid mobile phase.
- Volatile Compounds: Essential oils and aromatic terpenes are volatile and are separated/analyzed using Gas Chromatography (GC) or isolated via steam distillation.
Step 3: Final Answer:
The correct method is High-performance liquid chromatography (HPLC), which corresponds to option (A).
Analytical separation techniques are selected based on the volatility and thermal stability of the target chemical compounds.
Step 2: Detailed Explanation:
- Non-Volatile/Heat-Sensitive Compounds: These cannot be easily vaporized without chemical decomposition.
Therefore, Gas Chromatography (GC) and distillation methods cannot be used.
High-Performance Liquid Chromatography (HPLC) is the premier analytical technique used to separate and identify non-volatile compounds (such as capsaicin in chillies, curcumin in turmeric, and piperine in black pepper) dissolved in a liquid mobile phase.
- Volatile Compounds: Essential oils and aromatic terpenes are volatile and are separated/analyzed using Gas Chromatography (GC) or isolated via steam distillation.
Step 3: Final Answer:
The correct method is High-performance liquid chromatography (HPLC), which corresponds to option (A).
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