Question

Which of the following assays enable multiplexed analysis of a dozen or more SNP markers simultaneously ?
Pyrosequencing
Luminex
SNaPshot
Taqman
Choose the correct answer from the options given below :

• A. A and B Only
• B. D and A Only
• C. B and C Only
• D. C and D Only

Hint:

Multiplex SNP analysis → Luminex & SNaPshot are key technologies.

Answer 1

The Correct Option is C

Concept: Single Nucleotide Polymorphism (SNP) analysis is widely used in forensic genetics. Multiplexing refers to the ability to analyze multiple genetic markers simultaneously in a single experiment.

Step 1: Understanding multiplexing.
Multiplex assays allow simultaneous detection of multiple SNPs, improving efficiency and reducing time in forensic analysis.

Step 2: Evaluating Luminex (B).
Luminex technology uses bead-based multiplexing and can analyze multiple SNP markers at once.
Thus, B is correct.

Step 3: Evaluating SNaPshot (C).
SNaPshot is a primer extension-based method that allows multiplex SNP genotyping in a single reaction.
Thus, C is correct.

Step 4: Evaluating other methods.

• Pyrosequencing – Typically used for sequencing, limited multiplexing.
• Taqman – Usually used for single or limited SNP detection.

Step 5: Final evaluation.
Thus, multiplex capability is best represented by Luminex and SNaPshot. Final Conclusion:
Hence, the correct answer is option (3).