Question:
Which of the following are True for modifying enzymes used in rD technology?
A. Alkaline phosphatase is used to attach a phosphate group
B. Termil deoxynucleotidyl transferase can remove termil phosphate group
C. Reverse transcriptase is used to generate cD
D. Polynucleotide kise is used to attach a phosphate group
Which of the following are True for modifying enzymes used in rD technology?
A. Alkaline phosphatase is used to attach a phosphate group
B. Termil deoxynucleotidyl transferase can remove termil phosphate group
C. Reverse transcriptase is used to generate cD
D. Polynucleotide kise is used to attach a phosphate group
A. Alkaline phosphatase is used to attach a phosphate group
B. Termil deoxynucleotidyl transferase can remove termil phosphate group
C. Reverse transcriptase is used to generate cD
D. Polynucleotide kise is used to attach a phosphate group
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D Modifying Enzymes Summary:
Alkaline Phosphatase = REMOVES $5'$ phosphate.
Polynucleotide Kise = ADDS $5'$ phosphate.
Termil Transferase (TdT) = ADDS $3'$ homopolymer tails.
Reverse Transcriptase = R $\to$ cD.
Alkaline Phosphatase = REMOVES $5'$ phosphate.
Polynucleotide Kise = ADDS $5'$ phosphate.
Termil Transferase (TdT) = ADDS $3'$ homopolymer tails.
Reverse Transcriptase = R $\to$ cD.
Updated On: Jul 28, 2026
- B and C Only
- A and C Only
- C and D Only
- B and D Only
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Verified By Collegedunia
The Correct Option is C
Solution and Explanation
Step 1: Understanding the Question:
The question asks to select the true functiol descriptions of nucleic acid modifying enzymes utilized in recombint D technology.
Step 2: Key Formula or Approach:
Review the biochemical functions of modifying enzymes in molecular cloning:
• Alkaline Phosphatase: Removes $5'$ phosphate groups from D/R to prevent self-ligation.
• Polynucleotide Kise (T4 PNK): Adds/attaches a phosphate group from ATP to the $5'$-OH end of nucleic acids.
• Termil Deoxynucleotidyl Transferase (TdT): Adds template-independent dNTPs to the $3'$-OH end of D.
• Reverse Transcriptase: Synthesizes complementary D (cD) using an R template.
Step 3: Detailed Explation:
• Statement A (False): Alkaline phosphatase *removes* (cleaves) $5'$ phosphate groups; it does not attach them.
• Statement B (False): Termil deoxynucleotidyl transferase (TdT) catalyzes non-template extension of nucleotides at $3'$-OH ends; it does not remove termil phosphate groups.
• Statement C (True): Reverse transcriptase synthesizes a complementary D (cD) strand from an R template, an essential step in RT-PCR and cD library construction.
• Statement D (True): Polynucleotide kise transfers the $\gamma$-phosphate of ATP to the $5'$-hydroxyl termil of single- or double-stranded D/R, ebling radio-labeling or subsequent ligation.
Step 4: Fil Answer:
Statements C and D are true, corresponding to Option (C).
The question asks to select the true functiol descriptions of nucleic acid modifying enzymes utilized in recombint D technology.
Step 2: Key Formula or Approach:
Review the biochemical functions of modifying enzymes in molecular cloning:
• Alkaline Phosphatase: Removes $5'$ phosphate groups from D/R to prevent self-ligation.
• Polynucleotide Kise (T4 PNK): Adds/attaches a phosphate group from ATP to the $5'$-OH end of nucleic acids.
• Termil Deoxynucleotidyl Transferase (TdT): Adds template-independent dNTPs to the $3'$-OH end of D.
• Reverse Transcriptase: Synthesizes complementary D (cD) using an R template.
Step 3: Detailed Explation:
• Statement A (False): Alkaline phosphatase *removes* (cleaves) $5'$ phosphate groups; it does not attach them.
• Statement B (False): Termil deoxynucleotidyl transferase (TdT) catalyzes non-template extension of nucleotides at $3'$-OH ends; it does not remove termil phosphate groups.
• Statement C (True): Reverse transcriptase synthesizes a complementary D (cD) strand from an R template, an essential step in RT-PCR and cD library construction.
• Statement D (True): Polynucleotide kise transfers the $\gamma$-phosphate of ATP to the $5'$-hydroxyl termil of single- or double-stranded D/R, ebling radio-labeling or subsequent ligation.
Step 4: Fil Answer:
Statements C and D are true, corresponding to Option (C).
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