Question

Which of the following are the correct components of PCR reaction?

A. Taq Polymerase
B. Buffer (5X)
C. Primers (F and R)
D. NTPs
E. \(\text{H}_{2}\text{O}\)

Choose the correct answer from the options given below:

• A. A, B, C and E \
• B. A, B, D and E \
• C. A, C, D and E \
• D. A, C, D and B

Hint:

Watch out for NTPs vs dNTPs! PCR needs dNTPs (Deoxy) to synthesize DNA. Standard NTPs are used for making RNA.

Answer 1

The Correct Option is A

Step 1: Concept Polymerase Chain Reaction (PCR) is an in vitro technique used to amplify specific target DNA sequences. It relies on biochemical reagents interacting dynamically inside a thermal cycler.

Step 2: Meaning Standard component acronyms: F and R primers stand for Forward and Reverse oligonucleotides. NTPs refers generally to ribonucleoside triphosphates (used for RNA synthesis), whereas DNA polymerases specifically require dNTPs (deoxynucleoside triphosphates).

Step 3: Analysis Let us review the necessary constituents options: * A (Taq Polymerase) is required to extend the growing DNA strand. * B (Buffer) is vital to keep pH and ionic strength optimal. * C (Forward and Reverse primers) provide the essential 3'-OH starting group. * D states NTPs instead of dNTPs. DNA synthesis requires deoxyribonucleotides (dATP, dCTP, dGTP, dTTP) rather than standard ribonucleotides. * E ($\text{H}_{2}\text{O}$) serves as the solvent matrix for the chemical reactions. Comparing the presented combinations, option (1) which lists A, B, C, and E includes valid baseline requirements.

Step 4: Conclusion Thus, option A provides the best correct subset selection. Final Answer: (A)