Question:
Which enzyme is required to produce protoplast?
Which enzyme is required to produce protoplast?
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A protoplast is a plant or microbial cell that has had its cell wall completely removed, leaving just the cell surrounded by its membrane.
Updated On: Jun 24, 2026
- Cellulase and Pectinase
- Pectinase and Zymase
- Protease
- Pepsin
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The Correct Option is A
Solution and Explanation
Concept:
A protoplast is a plant or microbial cell that has had its cell wall completely removed, leaving just the cell surrounded by its membrane. To make one, you have to digest away the cell wall using enzymes that target the wall's building blocks.
Step 1:
The plant cell wall is made mainly of cellulose, with pectin acting like a glue holding cells together. So you need enzymes that break exactly these two materials.
Step 2:
Cellulase digests cellulose and pectinase digests pectin. Used together they strip off the whole wall and release a clean protoplast.
Step 3:
The other options do not fit. Zymase ferments sugars, while protease and pepsin break down proteins — none of these attack the cellulose or pectin of the wall.
Answer: Option (1) — Cellulase and Pectinase.
A protoplast is a plant or microbial cell that has had its cell wall completely removed, leaving just the cell surrounded by its membrane. To make one, you have to digest away the cell wall using enzymes that target the wall's building blocks.
Step 1:
The plant cell wall is made mainly of cellulose, with pectin acting like a glue holding cells together. So you need enzymes that break exactly these two materials.
Step 2:
Cellulase digests cellulose and pectinase digests pectin. Used together they strip off the whole wall and release a clean protoplast.
Step 3:
The other options do not fit. Zymase ferments sugars, while protease and pepsin break down proteins — none of these attack the cellulose or pectin of the wall.
Answer: Option (1) — Cellulase and Pectinase.
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