Question:

Which are the symbiotic nitrogen-fixing bio-fertillizers?
A. Rhizobium
B. Azotobacter
C. Azospirillum
D. Azollae
Choose the correct answer from the options given below:

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Rhizobium + Legumes and Azolla + Anabaena are the "Dynamic Duos" of symbiotic nitrogen fixation.
Updated On: May 20, 2026
  • A and C only
  • B and D only
  • C and B only
  • D and A only
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The Correct Option is D

Solution and Explanation

Concept: Bio-fertilizers are classified based on their lifestyle. Symbiotic bio-fertilizers live in a mutually beneficial, physical association with a host plant to fix nitrogen.

Step 1:
Identifying Symbiotic Relationships.
Rhizobium (A) is the most famous symbiotic bacterium, forming nodules on the roots of leguminous plants. Azollae (D) (specifically the water fern Azolla in association with the cyanobacterium Anabaena azollae) is a symbiotic system widely used in rice paddies.

Step 2:
Analyzing Free-living/Associative Fixers.
Azotobacter (B) is a strictly free-living diazotroph in the soil. Azospirillum (C) is often termed "associative," as it lives near or on the surface of roots (rhizosphere) but does not form a specialized internal symbiotic organ like a nodule.

Step 3:
Conclusion.
Because A and D represent the true "symbiotic" category in this list, option (4) is the correct answer.
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